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Immunofluorescence analysis of ICAM-1 / CD54 was done on 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ICAM-1 / CD54 (1A29) Mouse Monoclonal Antibody (MA5407) at 1ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat lymph node stroma|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA5407 targets CD54 in FACS, IHC, and Neu applications and shows reactivity with Rat, Human and Mouse samples.
The MA5407 immunogen is rat lymph node stroma.
MA5407 detects CD54 which has a predicted molecular weight of approximately 56 kDa.
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thoracic epidural bupivacaine attenuates inflammatory response, intestinal lipid peroxidation, oxidative injury, and mucosal apoptosis induced by mesenteric ischemia/reperfusion.
MA5407 was used in immunohistochemistry to investigate the effectivenes of thoracic epidural anesthesia in mesenteric ischemia/reperfusion
|Bedirli N,Akyürek N,Kurtipek O,Kavutcu M,Kartal S,Bayraktar AC||Anesthesia and analgesia (113:1226)||2011|
NAD(P)H oxidase mediates angiotensin II-induced vascular macrophage infiltration and medial hypertrophy.
MA5407 was used in immunohistochemistry to study the role of NAD(P)H oxidase in angiotensin II-induced vascular macrophage infiltration and medial hypertrophy
|Liu J,Yang F,Yang XP,Jankowski M,Pagano PJ||Arteriosclerosis, thrombosis, and vascular biology (23:776)||2003|
BB2; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor; myD10; P3.58
BB2; CD54; ICAM; Icam-1; ICAM1; Ly-47; MALA-2; P3.58