|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Raji cells and spleen cells fused with NS1 cells.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5-10µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
For IHC (P), prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required.
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Interaction between adipose tissue stromal cells and gastric cancer cells in vitro.
MA1-19028 was used in immunohistochemistry to study the in vitro interaction between adipose tissue stromal cells and gastric cancer cells
|Nomoto-Kojima N,Aoki S,Uchihashi K,Matsunobu A,Koike E,Ootani A,Yonemitsu N,Fujimoto K,Toda S||Cell and tissue research (344:287)||2011|
Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.
MA1-19028 was used in immunohistochemistry to study the reciprocal regulation of morphogenesis in co-cultures of adipose tissue explants and MDCK cells
|Udo K,Aoki S,Uchihashi K,Kawasaki M,Matsunobu A,Tokuda Y,Ootani A,Toda S,Uozumi J||Kidney international (78:60)||2010|