Immunofluorescent analysis of CD54 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD54 monoclonal antibody (Product # MA5405) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||Mouse ICAM-1 (CD54)|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5405 targets CD54 in WB, ELISA, FACS, IA, IHC, ICC/IF and Neu applications and shows reactivity with mouse samples.
The MA5405 immunogen is mouse ICAM-1 (CD54).
MA5405 detects CD54 which has a predicted molecular weight of approximately 56 kDa.
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amelioration of ischemia-reperfusion injury with cyclic peptide blockade of ICAM-1.
MA5405 was used in blocking/activating experiment to investigate the efficiency of IP25 for the rescue of ischemia induced damage
|Merchant SH,Gurule DM,Larson RS||American journal of physiology. Heart and circulatory physiology (284:H1260)||2003|
Blocking pulmonary ICAM-1 expression ameliorates lung injury in established diet-induced pancreatitis.
MA5405 was used in blocking/activating experiment to clarify the effect of ICAM-1 expression on the pulmonary injury from pancreatitis
|Lundberg AH,Fukatsu K,Gaber L,Callicutt S,Kotb M,Wilcox H,Kudsk K,Gaber AO||Annals of surgery (233:213)||2001|
In vivo microscopy of murine islets of Langerhans: increased adhesion of transferred lymphocytes to islets depends on macrophage-derived cytokines in a model of organ-specific insulitis.
MA5405 was used in immunohistochemistry to investigate the mechanism for the lymphocyte adhesion to islets of Langerhans
|Ludwig R,Kretschmer M,Caspar G,Bojunga J,Oldenburg A,Schumm-Draeger P,Stegmüller M,von Minckwitz G,Usadel KH,Kusterer K||Immunology (98:111)||1999|