Immunofluorescent analysis of CD54/ICAM-1 (green) showing staining in the membrane of HUVEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD54 monoclonal antibody (Product # MA5-13021) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Clone||W-CAM-1 (Wehi-CAM-1 or 1H4)|
|Immunogen||Raji Burkitt lymphoma cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13021 targets CD54 in WB, FACS, FN, IF, and IHC (F) applications and shows reactivity with Human samples.
The MA5-13021 immunogen is raji Burkitt lymphoma cells.
ICAM-1 (intercellular adhesion molecule) has 7 potential N-linked glycosylation sites. It is a single chain glycoprotein of Ig supergene family, present on unstimulated endo-thelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Exosomes derived from Epstein-Barr virus-infected cells are internalized via caveola-dependent endocytosis and promote phenotypic modulation in target cells.
MA5-13021 was used in immunocytochemistry to study the role of caveolin-dependent endocytosis in the internalization of exosomes released from cells infected with EBV
|Nanbo A,Kawanishi E,Yoshida R,Yoshiyama H||Journal of virology (87:10334)||2013|
A new organotypic culture of adipose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells.
MA5-13021 was used in immunocytochemistry and immunohistochemistry to develop a new culture system for studying multiple cell types within adipose tissue
|Sonoda E,Aoki S,Uchihashi K,Soejima H,Kanaji S,Izuhara K,Satoh S,Fujitani N,Sugihara H,Toda S||Endocrinology (149:4794)||2008|
Intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 at the early stages of atherosclerosis in a rat model.
MA5-13021 was used in immunohistochemistry to study ICAM-1 and VCAM-1 expression during the early stages of atherosclerosis in a rat model
|Fotis L,Agrogiannis G,Vlachos IS,Pantopoulou A,Margoni A,Kostaki M,Verikokos C,Tzivras D,Mikhailidis DP,Perrea D||In vivo (Athens, Greece) (26:243)||2012|
Interaction between adipose tissue stromal cells and gastric cancer cells in vitro.
MA5-13021 was used in immunohistochemistry to study the in vitro interaction between adipose tissue stromal cells and gastric cancer cells
|Nomoto-Kojima N,Aoki S,Uchihashi K,Matsunobu A,Koike E,Ootani A,Yonemitsu N,Fujimoto K,Toda S||Cell and tissue research (344:287)||2011|
Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.
MA5-13021 was used in immunohistochemistry to study the reciprocal regulation of morphogenesis in co-cultures of adipose tissue explants and MDCK cells
|Udo K,Aoki S,Uchihashi K,Kawasaki M,Matsunobu A,Tokuda Y,Ootani A,Toda S,Uozumi J||Kidney international (78:60)||2010|