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Leinco Technologies
ICAM-1 (CD54) Monoclonal Antibody (BE29G1), Functional Grade, In Vivo GOLD™
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Product Details
C391-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BE29G1
Immunogen
Purified Recombinant Mouse ICAM-1
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
≥5.0 mg/mL
Amount
1 mg
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Specificity: Rat Anti-Mouse CD54 (Clone BE29G1) recognizes the (Mr 95 kD) cell surface glycoprotein known as intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).
Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥95% monomer by analytical SEC.
Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.
This antibody has been tested in the following applications: Western Blotting, Immunoprecipitation, Blocking.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Human rhinovirus receptor; Icam1; intercellular adhesion molecule 1 precursor; Ly 47; MALA2; myD10; sCD54; soluble CD54; Surface antigen of activated B cells; unnamed protein product
Gene Aliases: CD54; Icam-1; Ly-47; MALA-2
UniProt ID: (Mouse) P13597
Entrez Gene ID: (Mouse) 15894
immune effector process
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
acute inflammatory response to antigenic stimulus
T cell antigen processing and presentation
positive regulation of cellular extravasation
immune response
cell adhesion
leukocyte cell-cell adhesion
I-kappaB kinase/NF-kappaB signaling
regulation of cell shape
membrane to membrane docking
positive regulation of actin filament polymerization
cell adhesion mediated by integrin
cell-cell adhesion mediated by integrin
positive regulation of GTPase activity
adhesion of symbiont to host
positive regulation of nitric oxide biosynthetic process
positive regulation of vasoconstriction
receptor-mediated virion attachment to host cell
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
negative regulation of calcium ion transport
establishment of endothelial barrier
leukocyte adhesion to vascular endothelial cell
positive regulation of ERK1 and ERK2 cascade
cellular response to glucose stimulus
T cell extravasation
establishment of endothelial intestinal barrier
establishment of Sertoli cell barrier
cell-cell adhesion
regulation of ruffle assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
cellular response to beta-amyloid
positive regulation of leukocyte adhesion to vascular endothelial cell
cellular response to leukemia inhibitory factor
negative regulation of endothelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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