Invitrogen

ICAM-3 Monoclonal Antibody (MEM-04)

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ICAM-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04) Monoclonal antibody (Product # MA1-19030).

ICAM-3 Antibody in Immunohistochemistry (IHC) — Figure 3. Immunohistochemical images of ICAM-2 and ICAM-3 staining. Immunostaining with ICAM-2 (A and B) and ICAM-3 (C and D) for human normal conjunctiva (A and C) and pterygia (B and D). Scale bar = 200 mm.

Product Details

MA1-19030

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	10 µg/mL	-
Flow Cytometry (Flow)	1-4 µg/mL	-
Functional Assay (FN)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG2b, kappa
Class	Monoclonal
Type	Antibody
Clone	MEM-04
Immunogen	Leukocytes from a patient suffering from LGL type leukemia. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	4° C, do not freeze
RRID	AB_1072540

Product Specific Information

Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Functional Application: Blocking.

Target Information

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased Expressions of ICAM-2 and ICAM-3 in Pterygium.

Current eye research

Demiryürek S,Saracaloglu A,Kimyon S,Mete A,Eronat O,Temiz E,Nacarkahya G,Tunca ZS,Düzen B,Saygili O,Güngör K,Karakök M,Demiryürek AT

Published figure using ICAM-3 monoclonal antibody (Product # MA1-19030) in Immunohistochemistry

Sat Jun 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD50; CDw50; ICAM-3; ICAM-R; Intercellular adhesion molecule 3; intercellular adhesion molecule-3

Gene Aliases: CD50; CDW50; ICAM-R; ICAM3

UniProt ID: (Human) P32942

Entrez Gene ID: (Human) 3385

Function(s)

stimulatory C-type lectin receptor signaling pathway phagocytosis cell adhesion single organismal cell-cell adhesion extracellular matrix organization regulation of immune response

Process(es)

receptor binding integrin binding protein binding

ICAM-3 Monoclonal Antibody (MEM-04)

Product Details

MA1-19030

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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