This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation and Permeabilization Buffer Set and protocol.
Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 450 is an alternative for Pacific Blue™. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Ac2-300; AI255428; BHLHB26; C78922; cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID2; GIG8; helix-loop-helix protein ID2; Id-2; ID2; ID2A; ID2H; Idb2; Inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
Gene Aliases: AI255428; BHLHB26; C78922; GIG8; Id-2; ID2; ID2A; ID2H; Idb2
Molecular Function: transcription factor