Proteintech

IFITM1 Monoclonal Antibody (5B5E2)

IFITM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

IFITM1 Antibody (60074-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60074-1-IG ( IFITM1-Specific Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IFITM1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

60074-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 54 publications 54 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

View 10 publications 10 publications

Flow Cytometry (Flow)

Assay-dependent

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Virus

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5B5E2

Immunogen

IFITM1-Specific Fusion Protein Ag2320 (1-125 aa encoded by BC000897)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

60074-1-Ig is a mouse monoclonal antibody which specifically recognizes IFITM1 but not IFITM2 or IFITM3.

Immunogen sequence: MHKEEHEVA VLGAPPSTIL PRSTVINIHS ETSVPDHVVW SLFNTLFLNW CCLGFIAFAY SVKSRDRKMV GDVTGAQAYA STAKCLNIWA LILGILMTIG FILLLVFGSV TVYHIMLQII QEKRGY (1-125 aa encoded by B C000897)

Target Information

IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon-gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion.

Cell host & microbe

Klein S,Golani G,Lolicato F,Lahr C,Beyer D,Herrmann A,Wachsmuth-Melm M,Reddmann N,Brecht R,Hosseinzadeh M,Kolovou A,Makroczyova J,Peterl S,Schorb M,Schwab Y,Brügger B,Nickel W,Schwarz US,Chlanda P

Wed Apr 12 00:00:00 UTC 2023

Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunity.

The EMBO journal

Unali G,Crivicich G,Pagani I,Abou-Alezz M,Folchini F,Valeri E,Matafora V,Reisz JA,Giordano AMS,Cuccovillo I,Butta GM,Donnici L,D'Alessandro A,De Francesco R,Manganaro L,Cittaro D,Merelli I,Petrillo C,Bachi A,Vicenzi E,Kajaste-Rudnitski A

Mon May 15 00:00:00 UTC 2023

Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunity.

The EMBO journal

Mon May 15 00:00:00 UTC 2023

Prolonged Primary Rhinovirus Infection of Human Nasal Epithelial Cells Diminishes the Viral Load of Secondary Influenza H3N2 Infection via the Antiviral State Mediated by RIG-I and Interferon-Stimulated Genes.

Cells

Ong HH,Liu J,Oo Y,Thong M,Wang Y,Chow VT

Thu Apr 13 00:00:00 UTC 2023

Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.

Journal of virology

Lu AY,Gustin A,Newhouse D,Gale M

Wed May 31 00:00:00 UTC 2023

IFITM2 Presents Antiviral Response through Enhancing Type I IFN Signaling Pathway.

Viruses

Chen L,Li X,Deng Y,Bi Y,Yan Z,Yang Y,Zhang X,Li H,Xie J,Feng R

Tue Mar 28 00:00:00 UTC 2023

Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption.

iScience

Cooley A,Madhukaran S,Stroebele E,Colon Caraballo M,Wang L,Akgul Y,Hon GC,Mahendroo M

Fri Feb 17 00:00:00 UTC 2023

Caveats of Using Overexpression Approaches to Screen Cellular Host IFITM Proteins for Antiviral Activity.

Pathogens (Basel, Switzerland)

Meischel T,Fritzlar S,Villalón-Letelier F,Smith JM,Brooks AG,Reading PC,Londrigan SL

Mon Mar 27 00:00:00 UTC 2023

Innate immune regulation in HIV latency models.

Retrovirology

Olson RM,Gornalusse G,Whitmore LS,Newhouse D,Tisoncik-Go J,Smith E,Ochsenbauer C,Hladik F,Gale M

Fri Jul 08 00:00:00 UTC 2022

Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.

Frontiers in microbiology

Stott-Marshall RJ,Foster TL

Tue Mar 15 00:00:00 UTC 2022

Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.

Frontiers in microbiology

Stott-Marshall RJ,Foster TL

Tue Mar 15 00:00:00 UTC 2022

Eukaryotic Initiation Factor 5A2 Regulates Expression of Antiviral Genes.

Journal of molecular biology

Farache D,Liu L,Lee ASY

Mon May 30 00:00:00 UTC 2022

Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model.

Cell reports

Cheng Y,Medina A,Yao Z,Basu M,Natekar JP,Lang J,Sanchez E,Nkembo MB,Xu C,Qian X,Nguyen PTT,Wen Z,Song H,Ming GL,Kumar M,Brinton MA,Li MMH,Tang H

Tue May 31 00:00:00 UTC 2022

A novel domain within the CIL regulates egress of IFITM3 from the Golgi and reveals a regulatory role of IFITM3 on the secretory pathway.

Life science alliance

Zhong L,Song Y,Marziali F,Uzbekov R,Nguyen XN,Journo C,Roingeard P,Cimarelli A

Fri Jul 01 00:00:00 UTC 2022

The Role of IFITM Proteins in Tick-Borne Encephalitis Virus Infection.

Journal of virology

Chmielewska AM,Gómez-Herranz M,Gach P,Nekulova M,Bagnucka MA,Lipińska AD,Rychłowski M,Hoffmann W,Król E,Vojtesek B,Sloan RD,Bieńkowska-Szewczyk K,Hupp T,Ball K

Wed Jan 12 00:00:00 UTC 2022

The Role of IFITM Proteins in Tick-Borne Encephalitis Virus Infection.

Journal of virology

Chmielewska AM,Gómez-Herranz M,Gach P,Nekulova M,Bagnucka MA,Lipińska AD,Rychłowski M,Hoffmann W,Król E,Vojtesek B,Sloan RD,Bieńkowska-Szewczyk K,Hupp T,Ball K

Wed Jan 12 00:00:00 UTC 2022

FTO Suppresses STAT3 Activation and Modulates Proinflammatory Interferon-Stimulated Gene Expression.

Journal of molecular biology

McFadden MJ,Sacco MT,Murphy KA,Park M,Gokhale NS,Somfleth KY,Horner SM

Wed Mar 30 00:00:00 UTC 2022

Assessing Mitochondrial DNA Release into the Cytosol and Subsequent Activation of Innate Immune-related Pathways in Mammalian Cells.

Current protocols

Bryant JD,Lei Y,VanPortfliet JJ,Winters AD,West AP

Tue Feb 01 00:00:00 UTC 2022

Inhibitory and Stimulatory Effects of IL-32 on HIV-1 Infection.

Journal of immunology (Baltimore, Md. : 1950)

Nasser H,Takahashi N,Eltalkhawy YM,Reda O,Lotfi S,Nasu K,Sakuragi JI,Suzu S

Thu Sep 01 00:00:00 UTC 2022

Rapalogs downmodulate intrinsic immunity and promote cell entry of SARS-CoV-2.

bioRxiv : the preprint server for biology

Shi G,Chiramel AI,Li T,Lai KK,Kenney AD,Zani A,Eddy A,Majdoul S,Zhang L,Dempsey T,Beare PA,Kar S,Yewdell JW,Best SM,Yount JS,Compton AA

Tue Aug 30 00:00:00 UTC 2022

The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2.

Journal of virology

Winstone H,Lista MJ,Reid AC,Bouton C,Pickering S,Galao RP,Kerridge C,Doores KJ,Swanson CM,Neil SJD

Mon Apr 12 00:00:00 UTC 2021

RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers.

Frontiers in oncology

Abraham HG,Ulintz PJ,Goo L,Yates JA,Little AC,Bao L,Wu Z,Merajver SD

Tue Nov 07 00:00:00 UTC 2023

Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis.

Journal of immunology (Baltimore, Md. : 1950)

Torres-Odio S,Lei Y,Gispert S,Maletzko A,Key J,Menissy SS,Wittig I,Auburger G,West AP

Thu Apr 15 00:00:00 UTC 2021

Long Noncoding RNA IFITM4P Regulates Host Antiviral Responses by Acting as a Competing Endogenous RNA.

Journal of virology

Xiao M,Chen Y,Wang S,Liu S,Rai KR,Chen B,Li F,Li Y,Maarouf M,Chen JL

Wed Oct 13 00:00:00 UTC 2021

ETV7 limits antiviral gene expression and control of influenza viruses.

Science signaling

Froggatt HM,Harding AT,Chaparian RR,Heaton NS

Tue Jul 13 00:00:00 UTC 2021

Post-transcriptional regulation of antiviral gene expression by N6-methyladenosine.

Cell reports

McFadden MJ,McIntyre ABR,Mourelatos H,Abell NS,Gokhale NS,Ipas H,Xhemalçe B,Mason CE,Horner SM

Tue Mar 02 00:00:00 UTC 2021

IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.

International journal of molecular medicine

Xu X,Wu Y,Yi K,Hu Y,Ding W,Xing C

Tue Jun 01 00:00:00 UTC 2021

Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteraction.

Life science alliance

Franz S,Pott F,Zillinger T,Schüler C,Dapa S,Fischer C,Passos V,Stenzel S,Chen F,Döhner K,Hartmann G,Sodeik B,Pessler F,Simmons G,Drexler JF,Goffinet C

Thu Jul 01 00:00:00 UTC 2021

IFITM Proteins That Restrict the Early Stages of Respiratory Virus Infection Do Not Influence Late-Stage Replication.

Journal of virology

Meischel T,Fritzlar S,Villalon-Letelier F,Tessema MB,Brooks AG,Reading PC,Londrigan SL

Mon Sep 27 00:00:00 UTC 2021

Syncytia formation by SARS-CoV-2-infected cells.

The EMBO journal

Buchrieser J,Dufloo J,Hubert M,Monel B,Planas D,Rajah MM,Planchais C,Porrot F,Guivel-Benhassine F,Van der Werf S,Casartelli N,Mouquet H,Bruel T,Schwartz O

Tue Dec 01 00:00:00 UTC 2020

Syncytia formation by SARS-CoV-2-infected cells.

The EMBO journal

Buchrieser J,Dufloo J,Hubert M,Monel B,Planas D,Rajah MM,Planchais C,Porrot F,Guivel-Benhassine F,Van der Werf S,Casartelli N,Mouquet H,Bruel T,Schwartz O

Tue Dec 01 00:00:00 UTC 2020

LY6E Restricts Entry of Human Coronaviruses, Including Currently Pandemic SARS-CoV-2.

Journal of virology

Zhao X,Zheng S,Chen D,Zheng M,Li X,Li G,Lin H,Chang J,Zeng H,Guo JT

Mon Aug 31 00:00:00 UTC 2020

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

mBio

Ahi YS,Yimer D,Shi G,Majdoul S,Rahman K,Rein A,Compton AA

Tue Jan 21 00:00:00 UTC 2020

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

mBio

Ahi YS,Yimer D,Shi G,Majdoul S,Rahman K,Rein A,Compton AA

Tue Jan 21 00:00:00 UTC 2020

p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.

PLoS pathogens

Wang X,Wu Z,Li Y,Yang Y,Xiao C,Liu X,Xiang X,Wei J,Shao D,Liu K,Deng X,Wu J,Qiu Y,Li B,Ma Z

Thu Oct 01 00:00:00 UTC 2020

The mRNA Cap 2'-O-Methyltransferase CMTR1 Regulates the Expression of Certain Interferon-Stimulated Genes.

mSphere

Williams GD,Gokhale NS,Snider DL,Horner SM

Wed May 13 00:00:00 UTC 2020

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Buchrieser J,Degrelle SA,Couderc T,Nevers Q,Disson O,Manet C,Donahue DA,Porrot F,Hillion KH,Perthame E,Arroyo MV,Souquere S,Ruigrok K,Dupressoir A,Heidmann T,Montagutelli X,Fournier T,Lecuit M,Schwartz O

Fri Jul 12 00:00:00 UTC 2019

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Fri Jul 12 00:00:00 UTC 2019

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Fri Jul 12 00:00:00 UTC 2019

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Fri Jul 12 00:00:00 UTC 2019

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

International journal of molecular sciences

Winkler M,Wrensch F,Bosch P,Knoth M,Schindler M,Gärtner S,Pöhlmann S

Thu Aug 08 00:00:00 UTC 2019

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

International journal of molecular sciences

Winkler M,Wrensch F,Bosch P,Knoth M,Schindler M,Gärtner S,Pöhlmann S

Thu Aug 08 00:00:00 UTC 2019

Identifying key barriers in cationic polymer gene delivery to human T cells.

Biomaterials science

Olden BR,Cheng E,Cheng Y,Pun SH

Tue Feb 26 00:00:00 UTC 2019

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

Thu Dec 06 00:00:00 UTC 2018

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

Thu Dec 06 00:00:00 UTC 2018

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Science translational medicine

Selman M,Ou P,Rousso C,Bergeron A,Krishnan R,Pikor L,Chen A,Keller BA,Ilkow C,Bell JC,Diallo JS

Wed Jan 24 00:00:00 UTC 2018

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Science translational medicine

Selman M,Ou P,Rousso C,Bergeron A,Krishnan R,Pikor L,Chen A,Keller BA,Ilkow C,Bell JC,Diallo JS

Wed Jan 24 00:00:00 UTC 2018

Multi-modal Potentiation of Oncolytic Virotherapy by Vanadium Compounds.

Molecular therapy : the journal of the American Society of Gene Therapy

Selman M,Rousso C,Bergeron A,Son HH,Krishnan R,El-Sayes NA,Varette O,Chen A,Le Boeuf F,Tzelepis F,Bell JC,Crans DC,Diallo JS

Wed Jan 03 00:00:00 UTC 2018

Multi-modal Potentiation of Oncolytic Virotherapy by Vanadium Compounds.

Molecular therapy : the journal of the American Society of Gene Therapy

Selman M,Rousso C,Bergeron A,Son HH,Krishnan R,El-Sayes NA,Varette O,Chen A,Le Boeuf F,Tzelepis F,Bell JC,Crans DC,Diallo JS

Wed Jan 03 00:00:00 UTC 2018

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 UTC 2018

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 UTC 2018

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Frontiers in immunology