Proteintech

IFITM1 Monoclonal Antibody (5B5E2)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IFITM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60074-1-IG ( IFITM1-Specific Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IFITM1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 60074-1-IG (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

IFITM1 Antibody in Immunoprecipitation (IP) — IP result of anti-IFITM1-Specific (IP:60074-1-IG, 4ug; Detection:60074-1-IG 1:500) with HepG2 cells lysate 3600ug.

Product Details

60074-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:50,000	View 25 publications 25 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Flow Cytometry (Flow)	Assay-dependent	View 3 publications 3 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunocytochemistry (ICC/IF)	-	View 6 publications 6 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Virus
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	5B5E2
Immunogen	IFITM1-Specific Fusion Protein Ag2320 (1-125 aa encoded by BC000897) View immunogen
Conjugate	Unconjugated CoraLite488 CoraLite594
Form	Liquid
Concentration	1.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

60074-1-Ig is a mouse monoclonal antibody which specifically recognizes IFITM1 but not IFITM2 or IFITM3.

Immunogen sequence: MHKEEHEVA VLGAPPSTIL PRSTVINIHS ETSVPDHVVW SLFNTLFLNW CCLGFIAFAY SVKSRDRKMV GDVTGAQAYA STAKCLNIWA LILGILMTIG FILLLVFGSV TVYHIMLQII QEKRGY (1-125 aa encoded by B C000897)

Target Information

IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon-gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.

Frontiers in immunology

Xu-Yang Z,Pei-Yu B,Chuan-Tao Y,Wei Y,Hong-Wei M,Kang T,Chun-Mei Z,Ying-Feng L,Xin W,Ping-Zhong W,Chang-Xing H,Xue-Fan B,Ying Z,Zhan-Sheng J

Thu Mar 31 00:00:00 EDT 2022

Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.

Frontiers in immunology

Xu-Yang Z,Pei-Yu B,Chuan-Tao Y,Wei Y,Hong-Wei M,Kang T,Chun-Mei Z,Ying-Feng L,Xin W,Ping-Zhong W,Chang-Xing H,Xue-Fan B,Ying Z,Zhan-Sheng J

Thu Mar 31 00:00:00 EDT 2022

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

mBio

Ahi YS,Yimer D,Shi G,Majdoul S,Rahman K,Rein A,Compton AA

Tue Jan 21 00:00:00 EST 2020

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Frontiers in immunology

Wang B,Lam TH,Soh MK,Ye Z,Chen J,Ren EC

Fri Aug 16 00:00:00 EDT 2019

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Frontiers in immunology

Wang B,Lam TH,Soh MK,Ye Z,Chen J,Ren EC

Fri Aug 16 00:00:00 EDT 2019

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Frontiers in immunology

Wang B,Lam TH,Soh MK,Ye Z,Chen J,Ren EC

Fri Aug 16 00:00:00 EDT 2019

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

International journal of molecular sciences

Winkler M,Wrensch F,Bosch P,Knoth M,Schindler M,Gärtner S,Pöhlmann S

Thu Aug 08 00:00:00 EDT 2019

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Buchrieser J,Degrelle SA,Couderc T,Nevers Q,Disson O,Manet C,Donahue DA,Porrot F,Hillion KH,Perthame E,Arroyo MV,Souquere S,Ruigrok K,Dupressoir A,Heidmann T,Montagutelli X,Fournier T,Lecuit M,Schwartz O

Fri Jul 12 00:00:00 EDT 2019

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Fri Jul 12 00:00:00 EDT 2019

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

Thu Dec 06 00:00:00 EST 2018

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 EDT 2018

IFITM proteins inhibit HIV-1 protein synthesis.

Scientific reports

Lee WJ,Fu RM,Liang C,Sloan RD

Fri Sep 28 00:00:00 EDT 2018

A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.

Retrovirology

Roesch F,OhAinle M,Emerman M

Tue Mar 20 00:00:00 EDT 2018

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Science translational medicine

Selman M,Ou P,Rousso C,Bergeron A,Krishnan R,Pikor L,Chen A,Keller BA,Ilkow C,Bell JC,Diallo JS

Wed Jan 24 00:00:00 EST 2018

Multi-modal Potentiation of Oncolytic Virotherapy by Vanadium Compounds.

Molecular therapy : the journal of the American Society of Gene Therapy

Selman M,Rousso C,Bergeron A,Son HH,Krishnan R,El-Sayes NA,Varette O,Chen A,Le Boeuf F,Tzelepis F,Bell JC,Crans DC,Diallo JS

Wed Jan 03 00:00:00 EST 2018

Rapid reversal of innate immune dysregulation in blood of patients and livers of humanized mice with HCV following DAA therapy.

PloS one

Burchill MA,Roby JA,Crochet N,Wind-Rotolo M,Stone AE,Edwards MG,Dran RJ,Kriss MS,Gale M,Rosen HR

Tue Oct 24 00:00:00 EDT 2017

Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.

The EMBO journal

Monel B,Compton AA,Bruel T,Amraoui S,Burlaud-Gaillard J,Roy N,Guivel-Benhassine F,Porrot F,Génin P,Meertens L,Sinigaglia L,Jouvenet N,Weil R,Casartelli N,Demangel C,Simon-Lorière E,Moris A,Roingeard P,Amara A,Schwartz O

Wed Jun 14 00:00:00 EDT 2017

IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis.

JCI insight

Raposo RAS,de Mulder Rougvie M,Paquin-Proulx D,Brailey PM,Cabido VD,Zdinak PM,Thomas AS,Huang SH,Beckerle GA,Jones RB,Nixon DF

Thu Jan 12 00:00:00 EST 2017

The IFITMs Inhibit Zika Virus Replication.

Cell reports

Savidis G,Perreira JM,Portmann JM,Meraner P,Guo Z,Green S,Brass AL

Tue Jun 14 00:00:00 EDT 2016

Global analysis of DNA methylation in hepatocellular carcinoma by a liquid hybridization capture-based bisulfite sequencing approach.

Clinical epigenetics

Gao F,Liang H,Lu H,Wang J,Xia M,Yuan Z,Yao Y,Wang T,Tan X,Laurence A,Xu H,Yu J,Xiao W,Chen W,Zhou M,Zhang X,Chen Q,Chen X

Mon Aug 24 00:00:00 EDT 2015

Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment.

Journal of virology

Xie M,Xuan B,Shan J,Pan D,Sun Y,Shan Z,Zhang J,Yu D,Li B,Qian Z

Sun Mar 01 00:00:00 EST 2015

Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment.

Journal of virology

Xie M,Xuan B,Shan J,Pan D,Sun Y,Shan Z,Zhang J,Yu D,Li B,Qian Z

Sun Mar 01 00:00:00 EST 2015

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

The Journal of general virology

Zhang W,Zhang L,Zan Y,Du N,Yang Y,Tien P

Thu Jan 01 00:00:00 EST 2015

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

The Journal of general virology

Zhang W,Zhang L,Zan Y,Du N,Yang Y,Tien P

Thu Jan 01 00:00:00 EST 2015

IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.

Cellular microbiology

Zhu X,He Z,Yuan J,Wen W,Huang X,Hu Y,Lin C,Pan J,Li R,Deng H,Liao S,Zhou R,Wu J,Li J,Li M

Thu Jan 01 00:00:00 EST 2015

IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread.

Cell host & microbe

Compton AA,Bruel T,Porrot F,Mallet A,Sachse M,Euvrard M,Liang C,Casartelli N,Schwartz O

Wed Dec 10 00:00:00 EST 2014

IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread.

Cell host & microbe

Compton AA,Bruel T,Porrot F,Mallet A,Sachse M,Euvrard M,Liang C,Casartelli N,Schwartz O

Wed Dec 10 00:00:00 EST 2014

IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.

Retrovirology

Tartour K,Appourchaux R,Gaillard J,Nguyen XN,Durand S,Turpin J,Beaumont E,Roch E,Berger G,Mahieux R,Brand D,Roingeard P,Cimarelli A

Tue Nov 25 00:00:00 EST 2014

IFITM proteins inhibit entry driven by the MERS-coronavirus spike protein: evidence for cholesterol-independent mechanisms.

Viruses

Wrensch F,Winkler M,Pöhlmann S

Fri Sep 26 00:00:00 EDT 2014

Interferon induction of IFITM proteins promotes infection by human coronavirus OC43.

Proceedings of the National Academy of Sciences of the United States of America

Zhao X,Guo F,Liu F,Cuconati A,Chang J,Block TM,Guo JT

Tue May 06 00:00:00 EDT 2014

Amphotericin B increases influenza A virus infection by preventing IFITM3-mediated restriction.

Cell reports

Lin TY,Chin CR,Everitt AR,Clare S,Perreira JM,Savidis G,Aker AM,John SP,Sarlah D,Carreira EM,Elledge SJ,Kellam P,Brass AL

Wed Nov 27 00:00:00 EST 2013

The CD225 domain of IFITM3 is required for both IFITM protein association and inhibition of influenza A virus and dengue virus replication.

Journal of virology

John SP,Chin CR,Perreira JM,Feeley EM,Aker AM,Savidis G,Smith SE,Elia AE,Everitt AR,Vora M,Pertel T,Elledge SJ,Kellam P,Brass AL

Mon Jul 01 00:00:00 EDT 2013

IFITM1 is a tight junction protein that inhibits hepatitis C virus entry.

Hepatology (Baltimore, Md.)

Wilkins C,Woodward J,Lau DT,Barnes A,Joyce M,McFarlane N,McKeating JA,Tyrrell DL,Gale M

Fri Feb 01 00:00:00 EST 2013

IFITM1 is a tight junction protein that inhibits hepatitis C virus entry.

Hepatology (Baltimore, Md.)

Wilkins C,Woodward J,Lau DT,Barnes A,Joyce M,McFarlane N,McKeating JA,Tyrrell DL,Gale M

Fri Feb 01 00:00:00 EST 2013

Innate immune tolerance and the role of kupffer cells in differential responses to interferon therapy among patients with HCV genotype 1 infection.

Gastroenterology

Lau DT,Negash A,Chen J,Crochet N,Sinha M,Zhang Y,Guedj J,Holder S,Saito T,Lemon SM,Luxon BA,Perelson AS,Gale M

Fri Feb 01 00:00:00 EST 2013

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Cell

Brass AL,Huang IC,Benita Y,John SP,Krishnan MN,Feeley EM,Ryan BJ,Weyer JL,van der Weyden L,Fikrig E,Adams DJ,Xavier RJ,Farzan M,Elledge SJ

Thu Dec 24 00:00:00 EST 2009

Bioinformatics

Protein Aliases: 27-Sep; CD225; Dispanin subfamily A member 2a; DSPA2a; Interferon-induced protein 17; Interferon-induced transmembrane protein 1; Interferon-inducible protein 9-27; Leu-13 antigen

Gene Aliases: 9-27; CD225; DSPA2a; IFI17; IFITM1; LEU13

UniProt ID: (Human) P13164

Entrez Gene ID: (Human) 8519

Function(s)

ossification cell surface receptor signaling pathway negative regulation of cell proliferation response to virus negative regulation of cell migration response to interferon-gamma response to interferon-alpha response to interferon-beta negative regulation of viral genome replication positive regulation of osteoblast differentiation negative regulation of viral entry into host cell regulation of immune response defense response to virus type I interferon signaling pathway

Process(es)

receptor signaling protein activity protein binding

IFITM1 Monoclonal Antibody (5B5E2)

Product Details

60074-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!