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|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human IFN alpha 2b|
|Purification||Caprylic acid precipitation|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
M710B targets IFN alpha in ELISA applications and shows reactivity with Human samples.
The M710B immunogen is recombinant human IFN alpha 2b.
M710B detects IFN alpha which has a predicted molecular weight of approximately 19 kDa.
Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma; MIM 147570) is produced by mitogen- or antigen-stimulated T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA.
M710B was used in ELISA to evaluate the consistency of a non-competitive sandwich fluorescent ELISA across different laboratories
|Noble JE,Wang L,Cerasoli E,Knight AE,Porter RA,Gray E,Howe C,Hannes E,Corbisier P,Wang J,Wu L,Altieri I,Patriarca M,Hoffman A,Resch-Genger U,Ebert B,Voigt J,Shigeri Y,Vonsky MS,Konopelko LA,Gaigalas AK,Bailey MJ||Clinical chemistry and laboratory medicine (46:1033)||2008|
Measles virus-induced modulation of host-cell gene expression.
M710B was used in ELISA to study measles virus-induced modulation of host-cell gene expression by human peripheral blood mononuclear cells
|Bolt G,Berg K,Blixenkrone-Møller M||The Journal of general virology (83:1157)||2002|
Cytokines delivered by biodegradable microspheres promote effective suppression of human tumors by human peripheral blood lymphocytes in the SCID-Winn model.
M710B was used in ELISA to study the anti-tumor effect of cytokines
|Egilmez NK,Jong YS,Hess SD,Jacob JS,Mathiowitz E,Bankert RB||Journal of immunotherapy (Hagerstown, Md. : 1997) (23:190)||2000|
Detection and quantification of blood-derived CD8+ T lymphocytes secreting tumor necrosis factor alpha in response to HLA-A2.1-binding melanoma and viral peptide antigens.
M710B was used in ELISA to study the role of tumor necross factor alpha in immune responses to HLA-A2.1-binding melanoma and viral peptide antigens
|Herr W,Schneider J,Lohse AW,Meyer zum Büschenfelde KH,Wölfel T||Journal of immunological methods (191:131)||1996|
Endogenous IFN-alpha production by plasmacytoid dendritic cells exerts an antiviral effect on thymic HIV-1 infection.
M710B was used in immunohistochemistry to investigate the mechanism for the inhibition of HIV infection by plasmacytoid dendritic cells
|Gurney KB,Colantonio AD,Blom B,Spits H,Uittenbogaart CH||Journal of immunology (Baltimore, Md. : 1950) (173:7269)||2004|
IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-1/13; interferon alpha-D; interferon, alpha 1; interferon-alpha1; leIF D
IFL; IFN; IFN-ALPHA; IFN-alphaD; IFNA1; IFNA13; IFNA@