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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||human leukocyte interferon|
|Purification||Ion-exchange chromatography, Size-exclusion chromatography|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Specificity: Neutralizes human interferon alpha; binds to human IFN alpha with high affinity (Ka=10^9 M^-1).
Activity: Interferon was titrated with the use of the cytopathic effect inhibition assay, where 1 unit/ml of interferon was necessary to produce an endpoint of 50%. One neutralization unit is defined as the amount of serum required to neutralize one unit of human IFN alpha to an endpoint of 50%.
Purity is >95%. Endotoxin level <1 EU/ug.
Store at -20°C for short-term use (less than 6 months). For long-term storage, aliquot and store at -70°C or below for retention of full activity. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% BSA.
Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma; MIM 147570) is produced by mitogen- or antigen-stimulated T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-1/13; interferon alpha-D; interferon-alpha1; leIF D
IFL; IFN; IFN-ALPHA; IFN-alphaD; IFNA13; IFNA@
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Intracellular signaling cascade MAPKKK cascade NF-kappaB cascade JAK-STAT cascade Cell communication Ligand-mediated signaling Immunity and defense T-cell mediated immunity Macrophage-mediated immunity Interferon-mediated immunity Cytokine/chemokine mediated immunity Apoptosis Induction of apoptosis Inhibition of apoptosis