|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||human leukocyte interferon|
|Purification||Ion-exchange chromatography, Size-exclusion chromatography|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Specificity: Neutralizes human interferon alpha; binds to human IFN alpha with high affinity (Ka=10^9 M^-1).
Activity: Interferon was titrated with the use of the cytopathic effect inhibition assay, where 1 unit/ml of interferon was necessary to produce an endpoint of 50%. One neutralization unit is defined as the amount of serum required to neutralize one unit of human IFN alpha to an endpoint of 50%.
Purity is >95%. Endotoxin level <1 EU/ug.
Store at -20°C for short-term use (less than 6 months). For long-term storage, aliquot and store at -70°C or below for retention of full activity. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% BSA.
Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma; MIM 147570) is produced by mitogen- or antigen-stimulated T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Intracellular signaling cascade MAPKKK cascade NF-kappaB cascade JAK-STAT cascade Cell communication Ligand-mediated signaling Immunity and defense T-cell mediated immunity Macrophage-mediated immunity Interferon-mediated immunity Cytokine/chemokine mediated immunity Apoptosis Induction of apoptosis Inhibition of apoptosis