|Flow Cytometry (Flow)||1:100|
|Western Blot (WB)||1-3 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 24–161 of human IFN-gamma|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
710287 was successfully to detect IFN-gamma in Flow application.
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
IFN-gamma is a member of type II interferon family, and is a dimeric protein consisting of two 146 amino acid subunits. It is involved in immunoregulatory and anti-proliferative functions. It is a potent activator of macrophages, induces the production of cytokines, and upregulates the expression of class I and class II MHC antigens. Activation of IFN-gamma takes place through binding of IFN-gamma receptor I and II, and activating the JAK-STAT pathway. IFN-gamma does not show any homology with IFN-alpha or IFN-beta but human IFN-gamma shows about 40% sequence homology with mouse IFN-gamma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: boIFNG; IFG; IFI; IFN-gamma; IFNG; Immune interferon; Interferon gamma; interferon gamma type 2; interferon, gamma; interferon-gamma
Gene Aliases: IFG; IFI; IFNG
UniProt ID: (Human) P01579
Entrez Gene ID: (Human) 3458
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