|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 23–156 of rat interferon gamma|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
IFN-gamma is a member of type II interferon family, and is a dimeric protein consisting of two 146 amino acid subunits. It is involved in immunoregulatory and anti-proliferative functions. It is a potent activator of macrophages, induces the production of cytokines, and upregulates the expression of class I and class II MHC antigens. Activation of IFN-gamma takes place through binding of IFN-gamma receptor I and II, and activating the JAK-STAT pathway. IFN-gamma does not show any homology with IFN-alpha or IFN-beta but human IFN-gamma shows about 40% sequence homology with mouse IFN-gamma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.