|Tested species reactivity||Bovine, Dog, Horse, Goat, Human, Mustelid, Sheep, Pig|
|Published species reactivity||Pig, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Bovine IFN gamma|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 2 publications below|
MBOIFNG targets IFN gamma in ELISA, and FACS applications and shows reactivity with Bovine, Canine, Equine, Goat, Human, Mustelid, Ovine, and Porcine samples.
The MBOIFNG immunogen is recombinant Bovine IFN gamma.
MBOIFNG detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Interferon-gamma response of PBMC indicates productive pseudorabies virus (PRV) infection in swine.
MBOIFNG was used in ELISA to study the IFN-gamma response of PBMCs as an ex vivo marker of productive pseudorabies virus infection in swine
|Hoegen B,Saalmüller A,Röttgen M,Rziha HJ,Geldermann H,Reiner G,Pfaff E,Büttner M||Veterinary immunology and immunopathology (102:389)||2004|
Cellular immune responses of schistosomiasis patients are altered by human immunodeficiency virus type 1 coinfection.
MBOIFNG was used in ELISA to study the effect of HIV-1 co-infection on the cellular immune responses of schistosomiasis patients
|Mwinzi PN,Karanja DM,Colley DG,Orago AS,Secor WE||The Journal of infectious diseases (184:488)||2001|