M701B targets IFN gamma in ELISA, and Wester blot applications and shows reactivity with Human samples.
The M701B immunogen is recombinant human IFN gamma.
M701B detects IFN gamma which has a predicted molecular weight of approximately 16 kDa.
The M701B IFNg antibody (biotinylated conjugate of clone B133.5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M700A (clone 2G1). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody M701B (clone B133.5) and antibody M700A (clone 2G1) have successfully been used in combination with recombinant IFNg protein SIFNG in ELISA applications.
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g
Gene Aliases: IFG; IFI; IFNG
UniProt ID: (Human) P01579
Entrez Gene ID: (Human) 3458