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|Tested species reactivity||Pig|
|Published species reactivity||Pig, Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant porcine IFN gamma|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MP701B targets IFN gamma in ELISA and WB applications and shows reactivity with Porcine samples.
The MP701B immunogen is recombinant porcine IFN gamma.
MP701B detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.
The MP700 mouse IFNg antibody (biotinylated conjugate of clone P2C11) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MP700 (clone PTF6). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Time-course of antibody and cell-mediated immune responses to Porcine Reproductive and Respiratory Syndrome virus under field conditions.
MP701B was used in ELISA to follow antibody and cell-mediated immune responses to PRRSV infection in pigs living in a farm environment
|Dotti S,Guadagnini G,Salvini F,Razzuoli E,Ferrari M,Alborali GL,Amadori M||Research in veterinary science (94:510)||2013|
Characterization of the interferon-α response of pigs to the weaning stress.
MP701B was used in ELISA to investigate the characteristics of interferon alpha response in pigs with early weaning
|Razzuoli E,Villa R,Sossi E,Amadori M||Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research (31:237)||2011|
Enhanced antiviral activity against foot-and-mouth disease virus by a combination of type I and II porcine interferons.
MP701B was used in blocking/activating experiment to study the antiviral effects of interferons in pigs
|Moraes MP,de Los Santos T,Koster M,Turecek T,Wang H,Andreyev VG,Grubman MJ||Journal of virology (81:7124)||2007|