|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat IFN-gamma.|
|Storage buffer||PBS with 125mM trehalose|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody neutralizes natural and recombinant rat IFN-y. Cross-reactivity to human IFN-y is less than 0.01% of the homologous combination. Biological Activity: >2.5 x 10^4 neutralizing units/mg, where one neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit recombinant rat IFN-y (~150 pg) in an antiviral activity bioassay.
For immunohistochemistry applications, it is recommended that the reconstituted antibody be dialyzed against PBS to remove trehalose.
Storage and reconstitution: store the lyophilized antibody at 2-8°C. Reconstitute using 0.5 mL sterile distilled water. Upon reconstitution, the antibody may be stored at 2-8°C for up to several weeks. For maximal stability, apportion the reconstituted antibody into working aliquots and store at -20°C, avoiding repeated freeze/thaw cycles. 0.02% sodium azide may be added to prevent bacterial growth.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.