|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IFN gamma|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 12 publications below|
P700 targets IFN gamma in ELISA, and WB applications and shows reactivity with Human samples.
The P700 immunogen is recombinant human IFN gamma.
P700 detects IFN gamma which has a predicted molecular weight of approximately 16 kDa.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Epstein-Barr virus nuclear antigen 1-specific CD4+ T cells directly kill Epstein-Barr virus-carrying natural killer and T cells.
P700 was used in ELISA to study the effect of Epstein-Barr virus nuclear antigen 1-specific CD4+ T cells in EBV infection
|Demachi-Okamura A,Ito Y,Akatsuka Y,Tsujimura K,Morishima Y,Takahashi T,Kuzushima K||Cancer science (99:1633)||2008|
Altered CD45 isoform expression in C77G carriers influences cytokine responsiveness and adhesion properties of T cells.
P700 was used in ELISA to investigate the effect of CD45 isoform expression changes on T cell activity
|Windhagen A,Sönmez D,Hornig-Do HT,Kalinowsky A,Schwinzer R||Clinical and experimental immunology (150:509)||2007|
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
P700 was used in ELISA to study the role of type 1 cytokine in chronic hepatosplenic schistosomiasis
|Montenegro SM,Abath FG,Domingues AL,Melo WG,Morais CN,Coutinho EM,Mahanty S,Wynn TA||Infection and immunity (70:5903)||2002|
Modulation of an interleukin-12 and gamma interferon synergistic feedback regulatory cycle of T-cell and monocyte cocultures by Porphyromonas gingivalis lipopolysaccharide in the absence or presence of cysteine proteinases.
P700 was used in ELISA to study the modulation of an IL-12 and gamma interferon regulatory cycle in T cell/monocyte co-cultures by Porphyromonas gingivalis lipopolysaccharide
|Yun PL,DeCarlo AA,Collyer C,Hunter N||Infection and immunity (70:5695)||2002|
Interferon-beta increases the stimulatory capacity of monocyte-derived dendritic cells to induce IL-13, IL-5 and IL-10 in autologous T-cells.
P700 was used in ELISA to investigate the effect of interferon beta on the function of monocyte-derived dendritic cells
|Wiesemann E,Sönmez D,Heidenreich F,Windhagen A||Journal of neuroimmunology (123:160)||2002|
Glatiramer acetate (GA) induces IL-13/IL-5 secretion in naive T cells.
P700 was used in ELISA to study the role of glatiramer acetate in interleukin 13/interleukin 5 secretion in naive T cells
|Wiesemann E,Klatt J,Sönmez D,Blasczyk R,Heidenreich F,Windhagen A||Journal of neuroimmunology (119:137)||2001|
Human nasopharyngeal-associated lymphoreticular tissues. Functional analysis of subepithelial and intraepithelial B and T cells from adenoids and tonsils.
P700 was used in ELISA to characterize subepithelial and intraepithelial B and T cells from adenoids and tonsils
|Boyaka PN,Wright PF,Marinaro M,Kiyono H,Johnson JE,Gonzales RA,Ikizler MR,Werkhaven JA,Jackson RJ,Fujihashi K,Di Fabio S,Staats HF,McGhee JR||The American journal of pathology (157:2023)||2000|
Induction of cytokines and killer cell activities by cisplatin and 5-fluorouracil in head and neck cancer patients.
P700 was used in ELISA to study the induction of cytokines and killer cell activities by cisplatin and 5-fluorouracil in head and neck cancer patients
|Okamoto M,Ohe G,Oshikawa T,Nishikawa H,Furuichi S,Yoshida H,Sato M||Anti-cancer drugs (11:165)||2000|
Cytokine production in acute versus chronic human Schistosomiasis mansoni: the cross-regulatory role of interferon-gamma and interleukin-10 in the responses of peripheral blood mononuclear cells and splenocytes to parasite antigens.
P700 was used in ELISA to investigate the role of interferon gamma and interleukin 10 in human Schistosomiasis mansoni responses
|Montenegro SM,Miranda P,Mahanty S,Abath FG,Teixeira KM,Coutinho EM,Brinkman J,Gonçalves I,Domingues LA,Domingues AL,Sher A,Wynn TA||The Journal of infectious diseases (179:1502)||1999|
A sensitive ELISPOT assay to detect low-frequency human T lymphocytes.
P700 was used in ELISA to develop a new assay to detect human T lymphocyte subsets
|McCutcheon M,Wehner N,Wensky A,Kushner M,Doan S,Hsiao L,Calabresi P,Ha T,Tran TV,Tate KM,Winkelhake J,Spack EG||Journal of immunological methods (210:149)||1997|
Phenotypic and cytokine analysis of human peripheral blood gamma delta T cells expressing NK cell receptors.
P700 was used in ELISA to characterize NK cell receptors on human peripheral blood gamma delta T cells
|Battistini L,Borsellino G,Sawicki G,Poccia F,Salvetti M,Ristori G,Brosnan CF||Journal of immunology (Baltimore, Md. : 1950) (159:3723)||1997|
|Non-human primate||1 ug/ml||
Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates.
P700 was used in ELISA to investigate the effect of oral immunization with simian immunodeficiency virus p55gag and cholera toxin for mucosal IgA and systemic IgG immune responses in nonhuman primates
|Kubota M,Miller CJ,Imaoka K,Kawabata S,Fujihashi K,McGhee JR,Kiyono H||Journal of immunology (Baltimore, Md. : 1950) (158:5321)||1997|