|Tested species reactivity||Pig|
|Published species reactivity||Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant porcine IFN gamma|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 3 publications below|
PP700 targets IFN gamma in WB applications and shows reactivity with Porcine samples.
The PP700 immunogen is recombinant porcine IFN gamma.
PP700 detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunogenicity and protective efficacy of an elastase-dependent live attenuated swine influenza virus vaccine administered intranasally in pigs.
PP700 was used in ELISA to characterize an elastase-dependent live attenuated influenza vaccine against swine influenza virus
|Masic A,Lu X,Li J,Mutwiri GK,Babiuk LA,Brown EG,Zhou Y||Vaccine (28:7098)||2010|
Identification of classical swine fever virus protein E2 as a target for cytotoxic T cells by using mRNA-transfected antigen-presenting cells.
PP700 was used in ELISA to study the role of classical swine fever virus protein E2 in cytotoxic T cell effect
|Ceppi M,de Bruin MG,Seuberlich T,Balmelli C,Pascolo S,Ruggli N,Wienhold D,Tratschin JD,McCullough KC,Summerfield A||The Journal of general virology (86:2525)||2005|
Fibrocytes are potent stimulators of anti-virus cytotoxic T cells.
PP700 was used in ELISA to study the activation of anti-virus cytotoxic T cells
|Balmelli C,Ruggli N,McCullough K,Summerfield A||Journal of leukocyte biology (77:923)||2005|