|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 24–161 of human IFN-gamma|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
IFN-gamma is a member of type II interferon family, and is a dimeric protein consisting of two 146 amino acid subunits. It is involved in immunoregulatory and anti-proliferative functions. It is a potent activator of macrophages, induces the production of cytokines, and upregulates the expression of class I and class II MHC antigens. Activation of IFN-gamma takes place through binding of IFN-gamma receptor I and II, and activating the JAK-STAT pathway. IFN-gamma does not show any homology with IFN-alpha or IFN-beta but human IFN-gamma shows about 40% sequence homology with mouse IFN-gamma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.