Invitrogen

IFN gamma Monoclonal Antibody (4S.B3), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details

16 Published Figures

24 References

View all (120) IFN gamma antibodies

Datasheet

Datasheet

Product Image Gallery

IFN gamma Antibody — Fig. 6 Human mo-DCs, but not mo-Mac, are efficient inducers of effector cytotoxic CD8 + T cells. Purified DCs and macrophage from tumor ascites were cultured with allogeneic CellTrace Violet-stained naive CD8 + T cells for 7 days, in the absence ( a , b ) or presence ( c , d ) of naive CD4 + T cells autologous to CD8 + T cells. Expression of Granzyme A, Perforin, and IFN-gamma was assessed by intracellular flow cytometry. a , c Representative results of eight independent experiments. Gated on live CD8 + T cells. b Number of proliferating CD8 + T cells is shown. Symbols represent individual donors. N = 8. Median is shown. d Number of CD8 + T cells expressing effector molecules is shown. Symbols represent individual donors. N = 8. Median is shown. * p < 0.05, ** p < 0.01, Wilcoxon non-parametric test

IFN gamma Antibody in Flow Cytometry (Flow) — Intracellular staining of 5-hour unstimulated (left) or PMA/Ionomycin-stimulated (with Brefeldin A) (right) normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IFN gamma PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

IFN gamma Antibody in Cell Treatment — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IFN gamma clone 4S.B3 stains a subset of CD4+ T cells only after stimulation and does not stain CD19+ B cells regardless of stimulation. Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation and Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IFN gamma (clone 4S.B3). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.125 µg/test

Published Applications

Flow Cytometry (Flow)

See 24 publications below

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host / Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4S.B3

Conjugate

PE-Cyanine7

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_469682

Product Specific Information

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: The 4S.B3 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This 4S.B3 antibody is offered in 2 formats: - µg size: has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.