Invitrogen

IFN gamma Monoclonal Antibody (AN-18), Functional Grade, eBioscience™

Product Image Gallery

IFN gamma Antibody in ELISA (ELISA) — Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in mouse IFN gamma ELISPOT assay. Control well is medium alone.

Product Details

16-7313-81

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	1-4 µg/mL	View 17 publications 17 publications
Functional Assay (FN)	Assay-Dependent	View 3 publications 3 publications
Control (Ctrl)	Assay-Dependent	-

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AN-18
Conjugate	Functional Grade Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_469246

Product Specific Information

Description: The AN-18 antibody reacts with mouse interferon-gamma (IFN gamma), a 15-20 kDa cytokine secreted by Th1 cells.

Applications Reported: The AN-18 antibody has been reported for use in ELISA and ELISPOT assay. For neutralization of IFN gamma activity, the alternate clones R4-6A2 or XMG1.2 are recommended.

Applications Tested: The AN-18 antibody has been tested as the capture antibody in IFN gamma ELISPOT assay, in combination with the biotinylated R4-6A2 antibody for detection. A concentration of 1-4 µg/mL is recommended. For neutralization of IFN gamma activity, the alternate clones R4-6A2 or XMG1.2 are recommended.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neurokinin-1 Receptor Signaling Is Required for Efficient Ca²⁺ Flux in T-Cell-Receptor-Activated T Cells.

Cell reports

Morelli AE,Sumpter TL,Rojas-Canales DM,Bandyopadhyay M,Chen Z,Tkacheva O,Shufesky WJ,Wallace CT,Watkins SC,Berger A,Paige CJ,Falo LD,Larregina AT

16-7313 was used in Flow cytometry/Cell sorting to demonstrate an effect of the NK1R in T cells that is relevant for immunotherapies based on pro-inflammatory neuropeptides and its receptors.

Tue Mar 10 00:00:00 EDT 2020

Development of a SFTSV DNA vaccine that confers complete protection against lethal infection in ferrets.

Nature communications

Kwak JE,Kim YI,Park SJ,Yu MA,Kwon HI,Eo S,Kim TS,Seok J,Choi WS,Jeong JH,Lee H,Cho Y,Kwon JA,Jeong M,Maslow JN,Kim YE,Jeon H,Kim KK,Shin EC,Song MS,Jung JU,Choi YK,Park SH

16-7313 was used in an ELISpot assay to investigate the development of the SFTSV DNA vaccine in ferrets.

Fri Aug 23 00:00:00 EDT 2019

Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease.

PLoS neglected tropical diseases

Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH

16-7313 was used in an ELISpot assay to develop a recombinant adenovirus Ad-EVVLP for the treatment of hand, foot and mouth disease, providing a possible vaccine for future immunisation against the disease.

Wed Apr 01 00:00:00 EDT 2015

Immunotherapeutic intervention with oncolytic adenovirus in mouse mammary tumors.

Oncoimmunology

Gibson H,Munns S,Freytag S,Barton K,Veenstra J,Bettahi I,Bissonette J,Wei WZ

16-7313 was used in an ELISpot assay to investigate how oncolytic adenoviral therapy can be used for immunotherapeutic intervention in mouse mammary tumors.

Thu Jan 01 00:00:00 EST 2015

Identification of novel epitopes from human papillomavirus type 18 E7 that can sensitize PBMCs of multiple HLA class I against human cervical cancer.

Journal of translational medicine

Kim S,Chung HW,Lee KR,Lim JB

16-7313 was used in an ELISpot assay to identify which epitopes from subtype 18 human papillomavirus elicit the strongest specific immune response.

Thu Aug 21 00:00:00 EDT 2014

DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8(+) T cell responses.

Clinical and experimental vaccine research

Lee J,Kim J,Lee J,Shin SJ,Shin EC

16-7313 was used in an ELISpot assay to examine if DNA immunisation of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional T cell responses.

Tue Jul 01 00:00:00 EDT 2014

The kinetics of myelin antigen uptake by myeloid cells in the central nervous system during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Sosa RA,Murphey C,Ji N,Cardona AE,Forsthuber TG

16-7313 was used in an ELISpot assay to investigate the kinetics of myelin Ag uptake by CNS APCs during experimental autoimmune encephalomyelitis and in the naive CNS.

Sun Dec 15 00:00:00 EST 2013

n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells.

The Journal of nutrition

Monk JM,Hou TY,Turk HF,McMurray DN,Chapkin RS

16-7313 was used in Flow cytometry/Cell sorting to discern the effect of n3 PUFAs on the CD4+ T-cells of mice.

Sun Sep 01 00:00:00 EDT 2013

Neutrophils confer T cell resistance to myeloid-derived suppressor cell-mediated suppression to promote chronic inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Ryan SO,Johnson JL,Cobb BA

16-7313 was used in Functional assays to reveal long-term persistence of an inflammation-associated neutrophil cell population constitutively producing IFN-γ.

Wed May 15 00:00:00 EDT 2013

Discovering naturally processed antigenic determinants that confer protective T cell immunity.

The Journal of clinical investigation

Gilchuk P,Spencer CT,Conant SB,Hill T,Gray JJ,Niu X,Zheng M,Erickson JJ,Boyd KL,McAfee KJ,Oseroff C,Hadrup SR,Bennink JR,Hildebrand W,Edwards KM,Crowe JE,Williams JV,Buus S,Sette A,Schumacher TN,Link AJ,Joyce S

16-7313 was used in an ELISpot assay to define targets of protective TCD8 immunity within human pathogens that have complex proteomes.

Wed May 01 00:00:00 EDT 2013

The cytokines interleukin 27 and interferon-γ promote distinct Treg cell populations required to limit infection-induced pathology.

Immunity

Hall AO,Beiting DP,Tato C,John B,Oldenhove G,Lombana CG,Pritchard GH,Silver JS,Bouladoux N,Stumhofer JS,Harris TH,Grainger J,Wojno ED,Wagage S,Roos DS,Scott P,Turka LA,Cherry S,Reiner SL,Cua D,Belkaid Y,Elloso MM,Hunter CA

16-7313 was used in an ELISA assay to study the promotion of distinct Treg cell populations by the cytokines IL-27 and IFN-y, and the role they play in limiting infection induced pathology.

Fri Sep 21 00:00:00 EDT 2012

Inhibition of SOCS1-/- lethal autoinflammatory disease correlated to enhanced peripheral Foxp3+ regulatory T cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Collins EL,Jager LD,Dabelic R,Benitez P,Holdstein K,Lau K,Haider MI,Johnson HM,Larkin J

16-7313 was used in an ELISA assay to show that CD4(+)/SOCS1-KIR combined treatment can synergistically promote the long-term survival of perinatal lethal SOCS1(-/-) mice.

Thu Sep 01 00:00:00 EDT 2011

The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Paterson AM,Brown KE,Keir ME,Vanguri VK,Riella LV,Chandraker A,Sayegh MH,Blazar BR,Freeman GJ,Sharpe AH

16-7313 was used in an ELISA assay to suggest that the immunoinhibitory functions of this pathway may be particularly important during the later phases of diabetogenesis.

Mon Aug 01 00:00:00 EDT 2011

T-cell response to human papillomavirus type 58 L1, E6, And E7 peptides in women with cleared infection, cervical intraepithelial neoplasia, or invasive cancer.

Clinical and vaccine immunology : CVI

Chan PK,Liu SJ,Cheung TH,Yeo W,Ngai SM,Cheung JL,Chong P,Man S

16-7313 was used in an ELISpot assay to investigate the T-cell response to HPV-58 L1, E6, and E7 peptides among women.

Wed Sep 01 00:00:00 EDT 2010

Polyethylenimine-based siRNA nanocomplexes reprogram tumor-associated dendritic cells via TLR5 to elicit therapeutic antitumor immunity.

The Journal of clinical investigation

Cubillos-Ruiz JR,Engle X,Scarlett UK,Martinez D,Barber A,Elgueta R,Wang L,Nesbeth Y,Durant Y,Gewirtz AT,Sentman CL,Kedl R,Conejo-Garcia JR

16-7313 was used in an ELISpot assay to investigate the use of polyethylenimine-based nanoparticles for therapeutic antitumour immunity.

Sat Aug 01 00:00:00 EDT 2009

Antitumoral immune response by recruitment and expansion of dendritic cells in tumors infected with telomerase-dependent oncolytic viruses.

Cancer research

Edukulla R,Ramakrishna E,Woller N,Mundt B,Knocke S,Gürlevik E,Saborowski M,Malek N,Manns MP,Wirth T,Kühnel F,Kubicka S

16-7313 was used in an ELISpot assay to investigate the effect of virotherapy in conjunction with MIP-1alpha and Flt3L in treating primary tumours, showing that triggering dendritic cell and T-cell infiltration can enhance treatment.

Sun Feb 15 00:00:00 EST 2009

Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei.

BMC immunology

Whitlock GC,Lukaszewski RA,Judy BM,Paessler S,Torres AG,Estes DM

16-7313 was used in Immunodepletion to demonstrate the involvement of B220+ cells and IFN-gamma and TNF-alpha in host protection following heat-killed Burkholderia mallei vaccination.

Mon Sep 29 00:00:00 EDT 2008

Depletion of dendritic cells delays ovarian cancer progression by boosting antitumor immunity.

Cancer research

Huarte E,Cubillos-Ruiz JR,Nesbeth YC,Scarlett UK,Martinez DG,Buckanovich RJ,Benencia F,Stan RV,Keler T,Sarobe P,Sentman CL,Conejo-Garcia JR

16-7313 was used in Immuno-electron microscopy to investigate the role of CD11c(+)DEC205(+) dendritic cells in maintenance of ovarian cancer tumour vasculature.

Mon Sep 15 00:00:00 EDT 2008

Myelin-reactive type B T cells and T cells specific for low-affinity MHC-binding myelin peptides escape tolerance in HLA-DR transgenic mice.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura K,McLaughlin KA,Weissert R,Forsthuber TG

16-7313 was used in an ELISpot assay to investigate the mechanisms underlying the association of MHC genes with multiple sclerosis.

Mon Sep 01 00:00:00 EDT 2008

Pertussis toxin-induced cytokine differentiation and clonal expansion of T cells is mediated predominantly via costimulation.

Cellular immunology

Denkinger CM,Denkinger MD,Forsthuber TG

16-7313 was used in an ELISpot assay to discern that costimulation is the predominant mediator between pertussis toxin-induced cytokine differentiation and clonal expansion of T cells.

Thu Mar 01 00:00:00 EST 2007

Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice.

Journal of neuroimmunology

Kort JJ,Kawamura K,Fugger L,Weissert R,Forsthuber TG

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

Gene Aliases: Ifg; IFN-g; Ifng

UniProt ID: (Mouse) P01580

Entrez Gene ID: (Mouse) 15978

Molecular Function: cytokine interferon superfamily intercellular signal molecule