Description: The DB-1 monoclonal antibody reacts with rat interferon-gamma (IFN-gamma), a 15-20 kDa cytokine secreted by Th1 cells. For optimal detection of rat IFN-gamma by sandwich ELISA, a combination of purified DB-1 for capture and biotinylated polyclonal anti-rat IFN-gamma for detection is recommended. DB-1 is used for capture of rat IFN-gamma by ELISA.
Applications Reported: DB-1 has been reported for use in capture of rat IFN gamma by ELISA.
Applications Tested: The DB-1 (DB1) antibody has been tested as the capture antibody in a sandwich ELISA for analysis of rat IFNg (Interferon-gamma, IFN-g) in combination with the biotin anti-rat IFNg poly (13-7320) antibody for detection and recombinant rat IFNg (14-8315) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IFN-gamma; Interferon gamma
Gene Aliases: Ifng; IFNG2
UniProt ID: (Rat) P01581
Entrez Gene ID: (Rat) 25712