Invitrogen

IFN gamma Monoclonal Antibody (XMG1.2), APC-eFluor™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

40 Published Figures

57 References

View all (173) IFN gamma antibodies

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IFN gamma Antibody in Flow Cytometry (Flow) — Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.03 µg of Rat IgG1 kappa Isotype Control APC-eFluor® 780 (Product # 47-4301-80) (left) or Anti-Mouse IFN gamma APC-eFluor® 780 (right). Total cells were used for analysis.

IFN gamma Antibody in Immunohistochemistry (IHC) — Figure 4 Characterization of T cell population in the kidneys of 19 and 15-week-old female MRL-Fas lpr KSRP -/- mice compared to MRL-Fas lpr KSRP +/+ mice ( a ) RNA was isolated from the kidneys of 19-week-old female MRL-Fas lpr KSRP -/- mice (KO) and MRL-Fas lpr KSRP +/+ mice (WT). T cell lineage-specific transcription factor mRNA expression of T-bet, GATA3, RORgammaT and FoxP3 were analyzed by qRT-PCR and normalized to the mRNA expression of Pol2a. Data presented are the mean +- SEM of the relative mRNA expressions based on WT female 19 weeks (100%) 9 to 14 animals per genotype (*: p < 0.05; n.s. : not significantly different from WT, T -TEST). ( b ) Immunostaining was used to analyze intra-renal frequency of FoxP3, Ki67 and IFN-gamma producing cells in the kidneys of female 19-week-old MRL-Fas pr KSRP +/+ and MRL-Fas lpr KSRP -/- mice. Data presented are the mean +- SEM of the score with 10 animals per genotype (**: p < 0.01, *: p < 0.05 compared to WT, TTEST). Representative image of immunostaining with 40x magnification. ( c - e ) FACS was used to analyze intra-renal frequency of CD4 + , CD4 + -IFN-gamma + and FoxP3 + cells in the kidneys of female 15-week-old MRL-Fas lpr KSRP +/+ and MRL-Fas pr KSRP -/- mice. Data presented are the mean +- SEM of the relative cell numbers compared to MRL-Fas lpr KSRP +/+ mice (19 to 23 animals per genotype) (**: p < 0.01, n.s. : not significant, compared to WT, t -test).

IFN gamma Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IFN gamma clone XMG1.2 stains a minor subset of CD4+ T cells and a larger subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IFN gamma (clone XMG1.2). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

47-7311-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.06 µg/test	View 56 publications 56 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	XMG1.2
Conjugate	APC-eFluor™ 780 Alexa Fluor 488 Alexa Fluor 561 Alexa Fluor 660 Alexa Fluor 700 APC Biotin Brilliant UV 395 Brilliant UV 615 Brilliant UV 661 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 480 Brilliant Violet 605 Brilliant Violet 650 Brilliant Violet 711 Brilliant Violet 786 eFluor 450 eFluor 660 FITC Functional Grade PE PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Unconjugated
Excitation/Emission Max	756/785 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2688061

Product Specific Information

Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

A peptide encoded by pri-miRNA-31 represses autoimmunity by promoting T_reg differentiation.

EMBO reports

Zhou H,Lou F,Bai J,Sun Y,Cai W,Sun L,Xu Z,Liu Z,Zhang L,Yin Q,Zhang J,Gao Y,Wang Z,Niu L,Cai X,Deng S,Wang H,Xia L,Ginhoux F,Li Q,Wang H

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Wed May 04 00:00:00 EDT 2022

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Neoantigen vaccine-induced CD4 T cells confer protective immunity in a mouse model of multiple myeloma through activation of CD8 T cells against non-vaccine, tumor-associated antigens.

Journal for immunotherapy of cancer

Bekri S,Rodney-Sandy R,Gruenstein D,Mei A,Bogen B,Castle J,Levey D,Cho HJ

47-7311-82 was used in Flow cytometry/Cell sorting to indicate that activation of CD8 CTL against MOPC315-associated antigens not present in the vaccine is one of the major mechanisms of tumor immunity.

Tue Feb 01 00:00:00 EST 2022

T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.

JCI insight

Chun N,Ang RL,Chan M,Fairchild RL,Baldwin WM,Horwitz JK,Gelles JD,Chipuk JE,Kelliher MA,Pavlov VI,Li Y,Homann D,Heeger PS,Ting AT

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Wed Dec 22 00:00:00 EST 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Small Molecule Inhibitors Targeting Nuclear Factor κB Activation Markedly Reduce Expression of Interleukin-2, but Not Interferon-γ, Induced by Phorbol Esters and Calcium Ionophores.

International journal of molecular sciences

Tanaka Y,Nakao A,Miyake Y,Higashi Y,Tanigaki R,Kataoka T

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Fri Dec 03 00:00:00 EST 2021

YY1 regulation by miR-124-3p promotes Th17 cell pathogenicity through interaction with T-bet in rheumatoid arthritis.

JCI insight

Lin J,Tang J,Lin J,He Y,Yu Z,Jiang R,Yang B,Ou Q

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Nov 22 00:00:00 EST 2021

Knockout of the KH-Type Splicing Regulatory Protein Drives Glomerulonephritis in MRL-Fas^lpr Mice.

Cells

Schmidtke L,Meineck M,Saurin S,Otten S,Gather F,Schrick K,Käfer R,Roth W,Kleinert H,Weinmann-Menke J,Pautz A

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Immunohistochemistry

Sun Nov 14 00:00:00 EST 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Sep 23 00:00:00 EDT 2021

Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis.

Journal of immunology research

Lin Y,Dong Y,Gao Y,Shi R,Li Y,Zhou X,Liu W,Li G,Qi Y,Wu Y

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Trichinella spiralis Paramyosin Induces Colonic Regulatory T Cells to Mitigate Inflammatory Bowel Disease.

Frontiers in cell and developmental biology

Hao C,Wang W,Zhan B,Wang Z,Huang J,Sun X,Zhu X

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Mechanisms that allow vaccination against an oncolytic vesicular stomatitis virus-encoded transgene to enhance safety without abrogating oncolysis.

Scientific reports

AuYeung AWK,Mould RC,Stegelmeier AA,van Vloten JP,Karimi K,Woods JP,Petrik JJ,Wood GA,Bridle BW

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.

Nature communications

Huang Y,Guan Z,Dai X,Shen Y,Wei Q,Ren L,Jiang J,Xiao Z,Jiang Y,Liu D,Huang Z,Xu X,Luo Y,Zhao C

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

The CD94/NKG2A inhibitory receptor educates uterine NK cells to optimize pregnancy outcomes in humans and mice.

Immunity

Shreeve N,Depierreux D,Hawkes D,Traherne JA,Sovio U,Huhn O,Jayaraman J,Horowitz A,Ghadially H,Perry JRB,Moffett A,Sled JG,Sharkey AM,Colucci F

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Cellular & molecular immunology

Mulas F,Wang X,Song S,Nishanth G,Yi W,Brunn A,Larsen PK,Isermann B,Kalinke U,Barragan A,Naumann M,Deckert M,Schlüter D

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

AIM2 controls microglial inflammation to prevent experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Ma C,Li S,Hu Y,Ma Y,Wu Y,Wu C,Liu X,Wang B,Hu G,Zhou J,Yang S

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon May 03 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Fine particulate matter induces airway inflammation by disturbing the balance between Th1/Th2 and regulation of GATA3 and Runx3 expression in BALB/c mice.

Molecular medicine reports

Pang L,Yu P,Liu X,Fan Y,Shi Y,Zou S

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Generation of a Novel Oncolytic Vaccinia Virus Using the IHD-W Strain.

Human gene therapy

Shin J,Hong SO,Kim M,Lee H,Choi H,Kim J,Hong J,Kang H,Lee E,Lee S,Kong B,Kim M,Choi H,Kim S

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

High Ambient Temperature Aggravates Experimental Autoimmune Uveitis Symptoms.

Frontiers in cell and developmental biology

Pan S,Tan H,Chang R,Wang Q,Zhu Y,Chen L,Li H,Su G,Zhou C,Cao Q,Kijlstra A,Yang P

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Fri Apr 09 00:00:00 EDT 2021

C5aR1 Activation Drives Early IFN-γ Production to Control Experimental Toxoplasma gondii Infection.

Frontiers in immunology

Briukhovetska D,Ohm B,Mey FT,Aliberti J,Kleingarn M,Huber-Lang M,Karsten CM,Köhl J

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-γ.

Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade.

Immunity

Bonavita E,Bromley CP,Jonsson G,Pelly VS,Sahoo S,Walwyn-Brown K,Mensurado S,Moeini A,Flanagan E,Bell CR,Chiang SC,Chikkanna-Gowda CP,Rogers N,Silva-Santos B,Jaillon S,Mantovani A,Reis e Sousa C,Guerra N,Davis DM,Zelenay S

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Runx proteins mediate protective immunity against Leishmania donovani infection by promoting CD40 expression on dendritic cells.

PLoS pathogens

Akhtar MN,Mishra M,Yadav V,Yadav M,Gujar R,Lal S,Kumar R,Khatri N,Sen P

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses.

NPJ vaccines

Li G,Adam A,Luo H,Shan C,Cao Z,Fontes-Garfias CR,Sarathy VV,Teleki C,Winkelmann ER,Liang Y,Sun J,Bourne N,Barrett ADT,Shi PY,Wang T

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Tumors induce de novo steroid biosynthesis in T cells to evade immunity.

Nature communications

Mahata B,Pramanik J,van der Weyden L,Polanski K,Kar G,Riedel A,Chen X,Fonseca NA,Kundu K,Campos LS,Ryder E,Duddy G,Walczak I,Okkenhaug K,Adams DJ,Shields JD,Teichmann SA

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Fri Jul 17 00:00:00 EDT 2020

PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice.

PloS one

Hu Z,Yu P,Du G,Wang W,Zhu H,Li N,Zhao H,Dong Z,Ye L,Tian J

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Jun 22 00:00:00 EDT 2020

Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model.

International journal of molecular sciences

Yoon CH,Ryu JS,Hwang HS,Kim MK

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Jun 11 00:00:00 EDT 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress.

Cell death & disease

Kim KW,Ryu JS,Ko JH,Kim JY,Kim HJ,Lee HJ,Oh JH,Chung JH,Oh JY

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

TAGAP instructs Th17 differentiation by bridging Dectin activation to EPHB2 signaling in innate antifungal response.

Nature communications

Chen J,He R,Sun W,Gao R,Peng Q,Zhu L,Du Y,Ma X,Guo X,Zhang H,Tan C,Wang J,Zhang W,Weng X,Man J,Bauer H,Wang QK,Martin BN,Zhang CJ,Li X,Wang C

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Apr 20 00:00:00 EDT 2020

Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.

Cell reports

Cham LB,Torrez Dulgeroff LB,Tal MC,Adomati T,Li F,Bhat H,Huang A,Lang PA,Moreno ME,Rivera JM,Galkina SA,Kosikova G,Stoddart CA,McCune JM,Myers LM,Weissman IL,Lang KS,Hasenkrug KJ

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Apr 14 00:00:00 EDT 2020

BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice.

JCI insight

Radhakrishnan RK,Thandi RS,Tripathi D,Paidipally P,McAllister MK,Mulik S,Samten B,Vankayalapati R

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2020

Specific targeting of CD163⁺ TAMs mobilizes inflammatory monocytes and promotes T cell-mediated tumor regression.

The Journal of experimental medicine

Etzerodt A,Tsalkitzi K,Maniecki M,Damsky W,Delfini M,Baudoin E,Moulin M,Bosenberg M,Graversen JH,Auphan-Anezin N,Moestrup SK,Lawrence T

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

The ROP16III-dependent early immune response determines the subacute CNS immune response and type III Toxoplasma gondii survival.

PLoS pathogens

Tuladhar S,Kochanowsky JA,Bhaskara A,Ghotmi Y,Chandrasekaran S,Koshy AA

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

BTK signaling drives CD1d^hiCD5⁺ regulatory B-cell differentiation to promote pancreatic carcinogenesis.

Oncogene

Das S,Bar-Sagi D

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Nature communications

Cao Y,Trillo-Tinoco J,Sierra RA,Anadon C,Dai W,Mohamed E,Cen L,Costich TL,Magliocco A,Marchion D,Klar R,Michel S,Jaschinski F,Reich RR,Mehrotra S,Cubillos-Ruiz JR,Munn DH,Conejo-Garcia JR,Rodriguez PC

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Wed Mar 20 00:00:00 EDT 2019

CD8⁺ T cells promote cytokine responses to stress.

Cytokine

Clark SM,Song C,Li X,Keegan AD,Tonelli LH

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Hippo Kinases Mst1 and Mst2 Sense and Amplify IL-2R-STAT5 Signaling in Regulatory T Cells to Establish Stable Regulatory Activity.

Immunity

Shi H,Liu C,Tan H,Li Y,Nguyen TM,Dhungana Y,Guy C,Vogel P,Neale G,Rankin S,Feng Y,Peng J,Tao W,Chi H

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

SYK-CARD9 Signaling Axis Promotes Gut Fungi-Mediated Inflammasome Activation to Restrict Colitis and Colon Cancer.

Immunity

Malik A,Sharma D,Malireddi RKS,Guy CS,Chang TC,Olsen SR,Neale G,Vogel P,Kanneganti TD

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Sep 18 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Mon Jan 02 00:00:00 EST 2017

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

The Nuclear Orphan Receptor NR2F6 Is a Central Checkpoint for Cancer Immune Surveillance.

Cell reports

Hermann-Kleiter N,Klepsch V,Wallner S,Siegmund K,Klepsch S,Tuzlak S,Villunger A,Kaminski S,Pfeifhofer-Obermair C,Gruber T,Wolf D,Baier G

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Treatment with targeted vesicular stomatitis virus generates therapeutic multifunctional anti-tumor memory CD4 T cells.

Cancer gene therapy

Gao Y,Whitaker-Dowling P,Griffin JA,Bergman I

Published figure using IFN gamma monoclonal antibody (Product # 47-7311-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

Gene Aliases: Ifg; IFN-g; Ifng

UniProt ID: (Mouse) P01580

Entrez Gene ID: (Mouse) 15978

Molecular Function: cytokine interferon superfamily intercellular signal molecule

Function(s)

Process(es)

cytokine activity interferon-gamma receptor binding protein binding

IFN gamma Monoclonal Antibody (XMG1.2), APC-eFluor™ 780, eBioscience™

Product Details

47-7311-82

Flow Cytometry (Flow)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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