Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # AHR0321) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||IGF-1R/3T3 mouse fibroblasts transfected with human type 1 IGF-receptor cDNA.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||2µg per mg lysate|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with an epitope at aa 283-440 (exon 4-6).
Recommended positive controls include placenta or breast tissue. This antibody is not suitable for Western blot. Without BSA, this antibody can be used as a capture antibody in a sandwich ELISA.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
IP-MS enrichment of IGF1R (LFQ intensity): IGF1R was enriched 141-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and IGF1R antibody (Part No. AHR0321). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Metabolic syndrome and colorectal cancer: is hyperinsulinemia/insulin receptor-mediated angiogenesis a critical process?
AHR0321 was used in immunohistochemistry - paraffin section to discuss mechanisms that link metabolic syndrome to colorectal cancer risk
|Liu JJ,Druta M,Shibata D,Coppola D,Boler I,Elahi A,Reich RR,Siegel E,Extermann M||Journal of geriatric oncology (5:40)||2014|
Increased signalling of EGFR and IGF1R, and deregulation of PTEN/PI3K/Akt pathway are related with trastuzumab resistance in HER2 breast carcinomas.
AHR0321 was used in immunohistochemistry - paraffin section to investigate the contribution of the PI3K/Akt pathway to trastuzumab resistance
|Gallardo A,Lerma E,Escuin D,Tibau A,Muñoz J,Ojeda B,Barnadas A,Adrover E,Sánchez-Tejada L,Giner D,Ortiz-Martínez F,Peiró G||British journal of cancer (106:1367)||2012|
The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?
AHR0321 was used in immunohistochemistry - paraffin section to study the category subdivision of pT1a and pT1b in renal cell carcinoma
|Langner C,Ratschek M,Rehak P,Tsybrovskyy O,Zigeuner R||BJU international (95:310)||2005|