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Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # AHR0321) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||IGF-1R/3T3 mouse fibroblasts transfected with human type 1 IGF-receptor cDNA.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||2µg per mg lysate|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with an epitope at aa 283-440 (exon 4-6).
Recommended positive controls include placenta or breast tissue. This antibody is not suitable for Western blot. Without BSA, this antibody can be used as a capture antibody in a sandwich ELISA.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IGFIR, IGFR, CD221, JTK13
IGF-I receptor, insulin-like growth factor 1 receptor, insulin-like growth factor I receptor, soluble IGF1R variant 1, soluble IGF1R variant 2, CD221