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Western blot analysis of IGF-IR / IGF1 Receptor was performed by loading 30 µg of A431 (lane1), PC-3 (lane2), A549 (lane3), SK-O-V3 (lane4), HeLa (lane5), HEK-293 (lane6), MDA-MB-231 (lane7) and MCF7 (lane8) cell lysate using NuPAGE® Novex® 10% Bis-Tris gel (NP0301BOX), XCell SureLock™ Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. IGF-IR / IGF1 Receptor was detected at ~95 and 135 kDa using IGF-IR / IGF1 Receptor Mouse Monoclonal Antibody (396700) at 2-3 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (626520) at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (WP20005). Additional bands at 135 and 95 kDa represent the alpha and beta chains of a fully mature cell membrane-bound IGF1R.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse|
|Immunogen||Human IGF1R beta|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The ALK inhibitor ASP3026 eradicates NPM-ALK⁺ T-cell anaplastic large-cell lymphoma in vitro and in a systemic xenograft lymphoma model.
39-6700 was used in western blot to determine the effects of ASP3026treatment on NPM-ALK+ ALCL cell lines in vitro and on systemic lymphoma growth in vivo
|George SK,Vishwamitra D,Manshouri R,Shi P,Amin HM||Oncotarget (5:5750)||2014|
MicroRNA 96 is a post-transcriptional suppressor of anaplastic lymphoma kinase expression.
39-6700 was used in western blot to study regulation of anaplastic lymphoma kinase expression in cancer cells.
|Vishwamitra D,Li Y,Wilson D,Manshouri R,Curry CV,Shi B,Tang XM,Sheehan AM,Wistuba II,Shi P,Amin HM||The American journal of pathology (180:1772)||2012|
|Inhibition of IGF-IR tyrosine kinase induces apoptosis and cell cycle arrest in imatinib-resistant chronic myeloid leukaemia cells.||Shi P,Chandra J,Sun X,Gergely M,Cortes JE,Garcia-Manero G,Arlinghaus RB,Lai R,Amin HM||Journal of cellular and molecular medicine (14:1777)||2010|
CD221; IGF-I receptor; IGFIR; IGFR; insulin-like growth factor I receptor; JTK13; soluble IGF1R variant 1; soluble IGF1R variant 2
CD221; IGF1R; IGFIR; IGFR; JTK13