|Tested species reactivity||Human, Rabbit|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Inhibition Assays (IA)||1.5 µg/mL|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13812 targets IGF-1 Receptor alpha in IA and IP applications and shows reactivity with Human and Rabbit samples.
The MA5-13812 immunogen is iGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
IP-MS enrichment of IGF1R (LFQ intensity): IGF1R was enriched 169-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and IGF1R antibody (Part No. MA5-13812). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.