Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # MA5-13817) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Inhibition Assays (IA)||1.5 µg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:10-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13817 targets IGF-1 Receptor alpha in IA, ICC/IF, WB and IP applications and shows reactivity with Human and Mouse samples.
The MA5-13817 immunogen is iGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
IP-MS enrichment of IGF1R (LFQ intensity): IGF1R was enriched 213-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and IGF1R antibody (Part No. MA5-13817). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Low levels of insulin-like growth factor type 1 receptor expression at cancer cell membrane predict liver metastasis in Dukes' C human colorectal cancers.
MA5-13817 was used in western blot to evaluate the prognostic significance of insulin-like growth factor type 1 receptor expression in Dukes human colorectal cancers
|Nakamura M,Miyamoto S,Maeda H,Zhang SC,Sangai T,Ishii G,Hasebe T,Endoh Y,Saito N,Asaka M,Ochiai A||Clinical cancer research : an official journal of the American Association for Cancer Research (10:8434)||2004|
Aurintricarboxylic acid induces a distinct activation of the IGF-I receptor signaling within MDA-231 cells.
MA5-13817 was used in immunoprecipitation to study the role of aurintricarboxylic acid in a distinct activation of IGF-1R signaling within breast cancer cells
|Haimsohn M,Beery R,Karasik A,Kanety H,Geier A||Endocrinology (143:837)||2002|