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Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # MA5-13817) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Inhibition Assays (IA)||1.5 µg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:10-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13817 targets IGF-1 Receptor alpha in IA, ICC/IF, WB and IP applications and shows reactivity with Human and Mouse samples.
The MA5-13817 immunogen is iGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Low levels of insulin-like growth factor type 1 receptor expression at cancer cell membrane predict liver metastasis in Dukes' C human colorectal cancers.
MA5-13817 was used in western blot to evaluate the prognostic significance of insulin-like growth factor type 1 receptor expression in Dukes human colorectal cancers
|Nakamura M,Miyamoto S,Maeda H,Zhang SC,Sangai T,Ishii G,Hasebe T,Endoh Y,Saito N,Asaka M,Ochiai A||Clinical cancer research : an official journal of the American Association for Cancer Research (10:8434)||2004|
Aurintricarboxylic acid induces a distinct activation of the IGF-I receptor signaling within MDA-231 cells.
MA5-13817 was used in immunoprecipitation to study the role of aurintricarboxylic acid in a distinct activation of IGF-1R signaling within breast cancer cells
|Haimsohn M,Beery R,Karasik A,Kanety H,Geier A||Endocrinology (143:837)||2002|
CD221; IGF-I receptor; insulin-like growth factor 1 receptor; insulin-like growth factor I receptor; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2
A330103N21Rik; CD221; D930020L01; hyft; IGF-1R; IGF1R; IGFIR; IGFR; JTK13