Invitrogen

IGF1R alpha Monoclonal Antibody (24-60)

Advanced Verification

IP-mass spectrometry

3 Published Figures

View all (18) IGF1R alpha antibodies

Questions & Answers

Tech Support

Questions & Answers

Tech Support

Product Image Gallery

IGF1R alpha Antibody in Immunocytochemistry (ICC/IF) — Knockdown of Insulin-like growth factor 1 receptor was achieved by transfecting MCF7 cells with Insulin-like growth factor 1 receptor specific siRNA (Silencer® select Product # S7213, S7211). Immunofluorescence analysis was performed on untransfected MCF7 cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Insulin-like growth factor 1 receptor specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with IGF1R alpha Monoclonal Antibody (24-60) (Product # MA5-13817, 1:100) followed by Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:4000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (Red) staining. No signal was observed in siRNA slide of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Insulin-like growth factor 1 receptor (Green). The Images were captured at 60X magnification.

IGF1R alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Insulin-like growth factor 1 receptor was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IGF1R alpha Monoclonal Antibody (24-60) (Product # MA5-13817) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

IGF1R alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # MA5-13817) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

IGF1R alpha Antibody in Western Blot (WB) — Western blot analysis of IGF-1 Receptor alpha was performed by loading 25 µg of SK-BR-3 (lane 1) and C2C12 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with an IGF-1 Receptor alpha monoclonal antibody (Product # MA5-13817) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~155 kDa.

IGF1R alpha Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 1 Full length IGF-1Ralpha- and beta-subunits are detectable in the nucleus of prostate cancer cells a Domain structure of mature IGF-1R at the plasma membrane, showing extracellular alpha-subunit (green), transmembrane beta-subunit (red), extracellular (ED), transmembrane (TM), kinase domains (KD) and disulphide bonds, modified from []. b , c Western blots showing detection of both IGF-1Ralpha- and beta-subunits in the nucleus of DU145 ( b ) and 22Rv1 ( c ) cells. Cells were serum-starved for 24 h, treated with solvent (4.5 uM HCl) or IGF-1 (50 nM) for 20 min before fractionation. All western blots shown are representative of 3 independent biological repeats. Arrowheads: 220 kDa IGF-1R proreceptor. d Images showing immunofluorescence staining of IGF-1Ralpha and IGF-1Rbeta in DU145 cells. DU145 cells were serum-starved and IGF-treated as in b-c followed by immunofluorescent staining. Graphs: mean +- SD nuclear IGF-1Ralpha- and beta-subunits (n >= 30 cells per condition for n = 3 biological repeats). Data were analysed by unpaired student''s t test and quantification of nuclear levels of IGF-1Ralpha- and beta-subunits showed a significant increase on treatment with IGF-1 (*p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001) e Images showing IGF-1Ralpha and IGF-1Rbeta immunofluorescence in DU145 cells. Cells were grown in full medium with 10% FCS and incubated with solvent (0.1% DMSO) or IGF-1R inhibitor BMS-754807 (100 nM) for 6 h at 37 degC and processed for immunofluore

IGF1R alpha Antibody in Immunoprecipitation, ChIP assay (IP, ChIP) — Fig. 3 Recruitment of IGF-1Ralpha is detectable at IGF-1Rbeta DNA binding sites. a Images from the UCSC genome browser of previously obtained ChIP-seq data showing IGF-1R binding peaks that denote recruitment of RNAPolII and IGF-1Rbeta to the JUN promoter and IntChr17 [ref ]. b , c Quantification of recruitment of IGF-1Ralpha or IGF-1Rbeta vs IgG negative control to IGF-1Rbeta binding regions, detected by ChIP-qPCR. Results represent mean +- SEM of triplicate independent experiments, each with 3 technical replicates. Recruitment of both IGF-1Ralpha and IGF-1Rbeta vs IgG was detected at the JUN promoter ( b ) and putative IntChr17 enhancer (c; p* < 0.05, p** < 0.005, p**** < 0.0001, Tukey''s multiple comparisons test)

IGF1R alpha Antibody in Immunoprecipitation (IP) — Fig. 2 Nuclear IGF-1Ralpha- and beta-subunits originate from the cell surface and interact in the nucleus a, b Western blots showing detection of cell surface biotinylated IGF-1R in the nucleus of DU145 ( a ) and 22Rv1 ( b ) cells. Detection of cell surface biotinylated EGFR in nuclear fractions acts as a positive control. Western blots shown are representative of 3 independent biological repeats. c, d Co-immunoprecipitation Western blot showing interaction of IGF-1Ralpha- and beta-subunits in the nucleus of DU145 ( c ) and 22Rv1 ( d ) cells. Western blots shown are representative of 3 independent biological repeats

IGF1R alpha Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with Insulin-like growth factor 1 receptor siRNA and decrease in signal intensity was observed in ICC application using Anti-IGF1R alpha Monoclonal Antibody (24-60) (Product # MA5-13817). {KD}

IGF1R alpha Antibody — IP-MS enrichment of IGF1R (LFQ intensity): IGF1R was enriched 213-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and IGF1R antibody (Product # MA5-13817). See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

MA5-13817

Applications	Tested Dilution	Publications
Western Blot (WB)	1:10-1:200	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	2 µg/mL	View 2 publications 2 publications
ChIP assay (ChIP)	-	View 1 publication 1 publication
Inhibition Assays (IA)	1.5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	24-60
Immunogen	IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10986755

Product Specific Information

MA5-13817 targets IGF-1 Receptor alpha in IA, ICC/IF, WB and IP applications and shows reactivity with Human and Mouse samples.

The MA5-13817 immunogen is iGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.

Target Information

IGF1R (Insulin-like Growth Factor1 Receptor) is a receptor tyrosine kinase that mediates actions of insulin-like growth factor 1 (IFG1). It binds IGF1 with high affinity and IGF2/insulin with lower affinity. Activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survial of malignant cells. Ligand binding activates the receptor kinase and leads to receptor autophosphorylation and tyrosines phosphorylation of multiple substrates that function as signaling adapter proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

IGF-1R nuclear import and recruitment to chromatin involves both alpha and beta subunits.

Discover. Oncology

Mills JV,Osher E,Rieunier G,Mills IG,Macaulay VM

Published figure using IGF1R alpha monoclonal antibody (Product # MA5-13817) in Immunoprecipitation

Sat Apr 23 00:00:00 EDT 2022

Analysis of Increased EGFR and IGF-1R Signaling and Its Correlation with Socio-Epidemiological Features and Biological Profile in Breast Cancer Patients: A Study in Northern Brazil.

Breast cancer (Dove Medical Press)

Silva Rocha F,da Silva Maués JH,Brito Lins Pereira CM,Moreira-Nunes CA,Rodriguez Burbano RM

MA5-13817 was used in Immunohistochemistry (Paraffin) to to evaluate the prognostic and predictive values of the EGFR and IGF-1R in tumor samples from patients with BC and their correlation with socio-epidemiological features.

Sat Apr 23 00:00:00 EDT 2022

Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.

Genes

Liu Y,Wang Y,Yao D,Chen X,Zhang F,Feng Y,Li X

MA5-13817 was used in Western Blot to support the use of Diane-35 and metformin combination therapy for patients with PCOS and early EC.

Wed Jan 12 00:00:00 EST 2022

Low levels of insulin-like growth factor type 1 receptor expression at cancer cell membrane predict liver metastasis in Dukes' C human colorectal cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nakamura M,Miyamoto S,Maeda H,Zhang SC,Sangai T,Ishii G,Hasebe T,Endoh Y,Saito N,Asaka M,Ochiai A

MA5-13817 was used in western blot to evaluate the prognostic significance of insulin-like growth factor type 1 receptor expression in Dukes human colorectal cancers

Wed Dec 15 00:00:00 EST 2004

Aurintricarboxylic acid induces a distinct activation of the IGF-I receptor signaling within MDA-231 cells.

Endocrinology

Haimsohn M,Beery R,Karasik A,Kanety H,Geier A

MA5-13817 was used in immunoprecipitation to study the role of aurintricarboxylic acid in a distinct activation of IGF-1R signaling within breast cancer cells

Fri Mar 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: CD221; IGF-I receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2

Gene Aliases: A330103N21Rik; CD221; D930020L01; hyft; IGF-1R; IGF1R; IGFIR; IGFR; JTK13

UniProt ID: (Human) P08069, (Mouse) Q60751

Entrez Gene ID: (Human) 3480, (Mouse) 16001

Molecular Function: transmembrane signal receptor

immune response signal transduction positive regulation of cell proliferation insulin receptor signaling pathway phosphatidylinositol 3-kinase signaling positive regulation of cell migration peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of DNA replication regulation of JNK cascade protein autophosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway axonogenesis brain development epidermis development organ morphogenesis negative regulation of muscle cell apoptotic process phosphorylation establishment of cell polarity male sex determination mammary gland development exocrine pancreas development neuron projection development positive regulation of cytokinesis negative regulation of MAPK cascade positive regulation of MAPK cascade negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of mitotic nuclear division positive regulation of DNA metabolic process protein heterooligomerization positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling prostate gland epithelium morphogenesis positive regulation of steroid hormone biosynthetic process

Process(es)

protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding nucleotide binding G-protein alpha-subunit binding protein kinase activity transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups