|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant fragment of the cytoplasmic domain of human IGF-1R beta-subunit expressed in E. coli.|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
IP-MS enrichment of IGF1R (LFQ intensity): IGF1R was enriched 50-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and IGF1R antibody (Part No. AHO1292). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.