Western blot analysis of IGF-IR / IGF1 Receptor was performed by loading 30 µg of A431 (lane1), PC-3 (lane2), A549 (lane3), SK-O-V3 (lane4), HeLa (lane5), HEK-293 (lane6), MDA-MB-231 (lane7) and MCF7 (lane8) cell lysate using NuPAGE® Novex® 10% Bis-Tris gel (NP0301BOX), XCell SureLock™ Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. IGF-IR / IGF1 Receptor was detected at ~95 and 135 kDa using IGF-IR / IGF1 Receptor Mouse Monoclonal Antibody (396700) at 2-3 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (626520) at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (WP20005). Additional bands at 135 and 95 kDa represent the alpha and beta chains of a fully mature cell membrane-bound IGF1R.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse|
|Immunogen||Human IGF1R beta|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
IP-MS enrichment of IGF1R (LFQ intensity): IGF1R was enriched 509-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and IGF1R antibody (Part No. 39-6700). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK¿ T-cell lymphoma.
39-6700 was used in western blot to analyze NPM-ALK+ T-cell lymphoma and the transcription factors MZF1 and Ik-1 that downregulate IGF-IR expression
|Vishwamitra D,Curry CV,Alkan S,Song YH,Gallick GE,Kaseb AO,Shi P,Amin HM||Molecular cancer (14:null)||2015|
The ALK inhibitor ASP3026 eradicates NPM-ALK¿ T-cell anaplastic large-cell lymphoma in vitro and in a systemic xenograft lymphoma model.
39-6700 was used in western blot to determine the effects of ASP3026treatment on NPM-ALK+ ALCL cell lines in vitro and on systemic lymphoma growth in vivo
|George SK,Vishwamitra D,Manshouri R,Shi P,Amin HM||Oncotarget (5:5750)||2014|
MicroRNA 96 is a post-transcriptional suppressor of anaplastic lymphoma kinase expression.
39-6700 was used in western blot to study regulation of anaplastic lymphoma kinase expression in cancer cells.
|Vishwamitra D,Li Y,Wilson D,Manshouri R,Curry CV,Shi B,Tang XM,Sheehan AM,Wistuba II,Shi P,Amin HM||The American journal of pathology (180:1772)||2012|
|Inhibition of IGF-IR tyrosine kinase induces apoptosis and cell cycle arrest in imatinib-resistant chronic myeloid leukaemia cells.||Shi P,Chandra J,Sun X,Gergely M,Cortes JE,Garcia-Manero G,Arlinghaus RB,Lai R,Amin HM||Journal of cellular and molecular medicine (14:1777)||2010|