Invitrogen

IGF2 Monoclonal Antibody (8H1)

7 Published Figures

6 References

View all (23) IGF2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (7)

IGF2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

IGF2 Antibody (MA5-17096) in ICC/IF

Immunofluorescence analysis of HeLa cells using IGF2 monoclonal antibody (Product # MA5-17096) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

IGF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-17096

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8H1

Immunogen

Purified recombinant fragment of human IGF2 (amino acids: 25-180) expressed in E. Coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538567

Product Specific Information

MA5-17096 targets IGF2 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17096 immunogen is purified recombinant fragment of human IGF2 (amino acids: 25-180) expressed in E. Coli.

MA5-17096 detects IGF2 which has a predicted molecular weight of approximately 20.1kDa.

Target Information

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability.

Journal of neuro-oncology

Uhlmann EJ,Mackel CE,Deforzh E,Rabinovsky R,Brastianos PK,Varma H,Vega RA,Krichevsky AM

MA5-17096 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to discover whether miRNAs dysregulated in meningioma, and explore miRNA-associated pathways amenable for therapeutic interventions.

Wed Mar 01 00:00:00 UTC 2023

Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability.

Journal of neuro-oncology

Uhlmann EJ,Mackel CE,Deforzh E,Rabinovsky R,Brastianos PK,Varma H,Vega RA,Krichevsky AM

Wed Mar 01 00:00:00 UTC 2023

Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability.

Journal of neuro-oncology

Uhlmann EJ,Mackel CE,Deforzh E,Rabinovsky R,Brastianos PK,Varma H,Vega RA,Krichevsky AM

Wed Mar 01 00:00:00 UTC 2023

Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway.

Open medicine (Warsaw, Poland)

Yang M,Luo J,Zhang S,Huang Q,Cao Q

MA5-17096 was used in Western Blotting to analyze the RNA expression of circ_0113656, microRNA-188-3p (miR-188-3p), and insulin-like growth factor 2 (IGF2) by quantitative real-time polymerase chain reaction.

Tue Jul 18 00:00:00 UTC 2023

Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway.

Open medicine (Warsaw, Poland)

Yang M,Luo J,Zhang S,Huang Q,Cao Q

Tue Jul 18 00:00:00 UTC 2023

Sperm-carried IGF2 downregulated the expression of mitogens produced by Sertoli cells: A paracrine mechanism for regulating spermatogenesis?

Frontiers in endocrinology

Cannarella R,Mancuso F,Arato I,Lilli C,Bellucci C,Gargaro M,Curto R,Aglietti MC,La Vignera S,Condorelli RA,Luca G,Calogero AE

MA5-17096 was used in Western Blot to investigate whether the Insulin-like growth factor 2 protein is expressed in human spermatozoa and, if so, its possible role in the cross-talk between germ and Sertoli cells (SCs) during spermatogenesis.

Thu Dec 22 00:00:00 UTC 2022

PRC2 Heterogeneity Drives Tumor Growth in Medulloblastoma.

Cancer research

Yi J,Kim B,Shi X,Zhan X,Lu QR,Xuan Z,Wu J

MA5-17096 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to report that the histone H3K27 methyltransferase polycomb repressor complex 2 (PRC2) is often heterogeneous within individual SHH medulloblastoma tumors.

Tue Aug 16 00:00:00 UTC 2022

Non-islet Cell Hypoglycemia: Case Series and Review of the Literature.

Frontiers in endocrinology

Garla V,Sonani H,Palabindala V,Gomez-Sanchez C,Subauste J,Lien LF

MA5-17096 was used in Immunohistochemistry to present two cases of NICH with different outcomes.

Mon May 25 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: FLJ22066; FLJ44734; H-IGF-2; IGF; IGF-II; Insulin like growth factor; Insulin-like growth factor 2; insulin-like growth factor 2 (somatomedin A); Insulin-like growth factor II; insulin-like growth factor II (IGF-II); Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein; insulin-like growth factor type 2; MSA; Multiplication-stimulating activity; Multiplication-stimulating polypeptide; preptin; Somatomedin-A; T3M-11-derived growth factor

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Gene Aliases: AL033362; C11orf43; GRDF; IGF-2; IGF-II; IGF2; IGFII; M6pr; Mpr; Peg2; PP1446; PP9974; RNIGF2

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UniProt ID: (Human) P01344, (Rat) P01346, (Mouse) P09535

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Entrez Gene ID: (Human) 3481, (Rat) 24483, (Mouse) 16002

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Function(s)

insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding growth factor activity receptor activator activity protein serine/threonine kinase activator activity growth factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IGF2 Monoclonal Antibody (8H1)

IGF2 Antibody (MA5-17096) in ICC/IF

Product Details

MA5-17096

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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