|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant, full length, human IGFBP3 protein.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||3-5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||8-25 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20185 detects IGFBP3 in human samples.
MA1-20185 has been successfully used in ELISA, immunohistochemistry (paraffin), and Western blot procedures. MA1-20185 detects a band of ~41 kDa.
The MA1-20185 immunogen is recombinant, full length, human IGFBP3 protein.
Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four lowaffinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. The 265 amino acid residue recombinant mature human IGFBP3 has a calculated molecular mass of approximately 29 kDa. Due to glycosylation, the recombinant protein migrates as 41 kDa in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.