|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein - full length mouse IkB alpha protein|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1500-1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28845 detects IKB alpha from mouse samples.
PA1-28845 has been successfully used in Western blot applications.
The PA1-28845 immunogen is recombinant protein - full length mouse IkB alpha protein.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
IKBa protein is a NF-kB inhibitory protein. It binds NF-kB, retains the complex in the cytoplasm, prevents it from entering nuclear and functioning as a transcription factor. Upon receiving a variety of signals, IKBa undergoes phosphorylation at serine residues by a ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds to DNA and affects gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.