|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cells.
I kappa B kinase alpha (IKK alpha) is a component of a multiprotein kinase complex that regulates the activity of the transcription factor NF-kappa B. Activation of the IKK complex via upstream stimuli leads to phosphorylation and degradation of NF-kappaB bound I kappa B (Inhibitor of kappa B). Subsequently, free NF-kappaB dimers enter the nucleus and regulate the transcription of a variety of target genes involved in cell proliferation and differentiation, apoptosis, and inflammation, etc. The IKK complex consists of 2 highly homologous kinase subunits, IKK alpha and IKK beta, and a nonenzymatic regulatory component, IKK gamma/NEMO. The relative contributions of IKK alpha and IKK beta to the signaling complex vary according to the requirements of the cell. IKK beta plays a predominant role in immune responses, while IKK alpha alone appears to be sufficient for at least some developmental systems. Recently, IKK/NF-kappa B signaling pathway was studied as therapeutic targets in cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.