This Antibody was verified by KnockOut to ensure that the antibody binds to the antigen stated. View Details
Suggested positive control: Daudi, NIH-3T3, or Raw.
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK alpha, IKK beta, and IKK gamma. In vitro, IKK alpha and IKK beta can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK alpha and IKK beta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1) (1), respectively. Targeted disruption of IKK alpha gene in mice results in skin and limb abnormalities and death of newborns (2,3).
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Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKBKA; IKK alpha; IKK-A; IKK-a kinase; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; TCF16; Transcription factor 16
Gene Aliases: AI256658; CHUK; Chuk1; Fbx24; Fbxo24; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
UniProt ID: (Human) O15111