Invitrogen

IKK epsilon Monoclonal Antibody (T.546.3)

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IKK epsilon Antibody in Western Blot (WB) — Western blot was performed using Anti-IKK epsilon Monoclonal Antibody (T.546.3) (Product # MA5-14817) and an 80 kDa band corresponding to IKK epsilon was observed in all cell lines tested. Expression of IKK epsilon was higher in MDA-MB-231 as compared to MCF 10A [10.1016/j.cell.2007.03.052]. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF 10A (Lane 2) and T98G (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) was also observed at ~28 kDa.

Product Details

MA5-14817

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunoprecipitation (IP)	1:100	-

Product Specifications
Species Reactivity	Human, Non-human primate
Host/Isotype	Rabbit / IgG
Class	Monoclonal
Type	Antibody
Clone	T.546.3
Immunogen	Synthetic peptide corresponding to residues surrounding Val345 of human IKK-epsilon View antigen
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	<0.02% sodium azide
Storage conditions	-20°C
RRID	AB_10985738

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other IKKs or with TBK1/NAK.

Target Information

Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: I-kappa-B kinase epsilon; IKK-epsilon; IKK-i; IKK-related kinase epsilon; Inducible I kappa-B kinase; inducible IkappaB kinase; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; RP11-534L20.1

Gene Aliases: IKBKE; IKK-E; IKK-i; IKKE; IKKI; KIAA0151

UniProt ID: (Human) Q14164

Entrez Gene ID: (Human) 9641

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme