Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | - |
Immunoprecipitation (IP) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Non-human primate |
Host/Isotype |
Rabbit / IgG |
Class |
Monoclonal |
Type |
Antibody |
Clone |
T.546.3 |
Immunogen |
Synthetic peptide corresponding to residues surrounding Val345 of human IKK-epsilon |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Affinity chromatography |
Storage buffer |
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
Contains |
<0.02% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_10985738 |
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other IKKs or with TBK1/NAK.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: I-kappa-B kinase epsilon; IKK-epsilon; IKK-i; IKK-related kinase epsilon; Inducible I kappa-B kinase; inducible IkappaB kinase; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; RP11-534L20.1
Gene Aliases: IKBKE; IKK-E; IKK-i; IKKE; IKKI; KIAA0151
UniProt ID: (Human) Q14164
Entrez Gene ID: (Human) 9641
Molecular Function:
non-receptor serine/threonine protein kinase
protein modifying enzyme
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