Western blot analysis of IKK-gamma in extracts from HeLa cells, untreated or treated with TNF-alpha (20 ng/ml) and a phosphatase inhibitor (50 nM), using IKK-gamma monoclonal antibody (Product # MA5-15155) (bottom) and a Phospho-IKK-gamma pSer376 polyclonal antibody (top).
|Tested species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length human GST-IKK gamma protein.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.