Invitrogen

IKK gamma Polyclonal Antibody

View all (39) IKK gamma antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IKK gamma Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of IKK gamma in C6 cells using a IKK gamma Polyclonal antibody (Product # PA5-120057). Blue: DAPI for nuclear staining.

IKK gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IKK gamma in human esophageal cancer using a Polyclonal antibody (Product # PA5-120057) at a dilution of 1:100.

IKK gamma Antibody in Western Blot (WB) — Western Blot analysis of IKK gamma in Rat brain using a IKK gamma Polyclonal antibody (Product # PA5-120057) at a dilution of 1:3,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Product Details

PA5-120057

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50 - 1:100	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A recombinant fusion protein containing a sequence corresponding to amino acids 250-390 of human IKK gamma. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.58 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.01% thimerosal
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2913629

Product Specific Information

Positive test controls include: HeLa, Jurkat, Mouse brain, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus.

Target Information

IKK gamma is the regulatory subunit of the inhibitor of the kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: FIP-3; gikk; I-kappa-B kinase gamma; I-kappa-B kinase subunit gamma; ikB kinase subunit gamma; IkB kinase-associated protein 1; IKK-gamma; IKK3; IKKAP1; IKKG; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of kappaB kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; mF; mFIP-3; NEMO; NF-kappa B essential modulator; NF-kappa-B essential modifier; NF-kappa-B essential modulator; OTTHUMP00000207079; OTTHUMP00000212188

Gene Aliases: 1110037D23Rik; AI848108; AI851264; AMCBX1; AW124339; FIP-3; FIP3; Fip3p; IKBKG; IKK-gamma; IKK[g]; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9

UniProt ID: (Human) Q9Y6K9, (Mouse) O88522, (Rat) Q6TMG5

Entrez Gene ID: (Human) 8517, (Mouse) 16151, (Rat) 309295

Function(s)

B cell homeostasis transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus activation of NF-kappaB-inducing kinase activity positive regulation of gene expression positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of transcription from RNA polymerase II promoter positive regulation of NF-kappaB transcription factor activity establishment of vesicle localization negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway activation of MAPK activity stimulatory C-type lectin receptor signaling pathway apoptotic process inflammatory response immune response I-kappaB kinase/NF-kappaB signaling JNK cascade response to virus regulation of tumor necrosis factor-mediated signaling pathway TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway innate immune response T cell receptor signaling pathway stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway negative regulation of neuron death NF-kappaB import into nucleus

Process(es)

signal transducer activity protein binding protein domain specific binding ubiquitin protein ligase binding protein homodimerization activity peroxisome proliferator activated receptor binding metal ion binding protein heterodimerization activity K63-linked polyubiquitin binding linear polyubiquitin binding

IKK gamma Polyclonal Antibody

Product Details

PA5-120057

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!