|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 400 to 416 of human IKK gamma|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot applications, this antibody detects a band at ~52kDa. A suggested positive control is Hela cell lysate.
PA5-19965 can be used with blocking peptide PEP-0090.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. The Ikappa-B kinase (IKK-a and IKK-b) phosphorylates IkB and mediates NF-kappa-B activation. A novel molecule in the IKK complex was recently identified and termed IKK-gamma/NEMO/FIP3/IKKAP1. IKK-gamma interacts with IKK-b and is required for the activation of IKK complex and NF-kappa-B by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kappa-B activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.