In Western blot applications, this antibody detects a band at ~52kDa. A suggested positive control is Hela cell lysate.
PA5-19965 can be used with blocking peptide PEP-0090.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. The Ikappa-B kinase (IKK-a and IKK-b) phosphorylates IkB and mediates NF-kappa-B activation. A novel molecule in the IKK complex was recently identified and termed IKK-gamma/NEMO/FIP3/IKKAP1. IKK-gamma interacts with IKK-b and is required for the activation of IKK complex and NF-kappa-B by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kappa-B activation.
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Protein Aliases: AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO; FIP-3; I-kappa-B kinase gamma; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; ikB kinase subunit gamma; IkB kinase-associated protein 1; IKK-gamma; IKKAP1; IKKG; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of kappaB kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; mFIP-3; NEMO; NF-kappa B essential modulator; NF-kappa-B essential modifier; NF-kappa-B essential modulator; NFkappaB essential modulator
Gene Aliases: 1110037D23Rik; AI848108; AI851264; AMCBX1; AW124339; FIP-3; FIP3; Fip3p; IKBKG; IKK-gamma; IKK[g]; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9