Invitrogen

IKK gamma Recombinant Rabbit Monoclonal Antibody (JA11-83)

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Datasheet

Protocols

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IKK gamma Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of IKK gamma in Hela cells using a IKK gamma Monoclonal antibody (Product # MA5-32682) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

IKK gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IKK gamma of paraffin-embedded Mouse fallopian tube tissue using a IKK-gamma Monoclonal antibody (Product #MA5-32682). Counter stained with hematoxylin.

IKK gamma Antibody in Western Blot (WB) — Western blot was performed using Anti-IKK gamma Recombinant Rabbit Monoclonal Antibody (JA11-83) (Product # MA5-32682) and a 50 kDa band corresponding to Ikbkg was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), A-431 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

IKK gamma Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of IKK gamma in Hela cells using a IKK gamma Monoclonal Antibody (Product # MA5-32682) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Details

MA5-32682

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:400	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JA11-83
Immunogen	Recombinant protein within Human IKK gamma aa 1-286 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809959

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

IKK gamma is the regulatory subunit of the inhibitor of the kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FIP-3; gikk; I-kappa-B kinase gamma; I-kappa-B kinase subunit gamma; ikB kinase subunit gamma; IkB kinase-associated protein 1; IKK-gamma; IKK3; IKKAP1; IKKG; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of kappaB kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; mF; mFIP-3; NEMO; NF-kappa B essential modulator; NF-kappa-B essential modifier; NF-kappa-B essential modulator; OTTHUMP00000207079; OTTHUMP00000212188

Gene Aliases: 1110037D23Rik; AI848108; AI851264; AMCBX1; AW124339; FIP-3; FIP3; Fip3p; IKBKG; IKK-gamma; IKK[g]; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9

UniProt ID: (Human) Q9Y6K9, (Mouse) O88522, (Rat) Q6TMG5

Entrez Gene ID: (Human) 8517, (Mouse) 16151, (Rat) 309295

Function(s)

B cell homeostasis transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus activation of NF-kappaB-inducing kinase activity positive regulation of gene expression positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of transcription from RNA polymerase II promoter positive regulation of NF-kappaB transcription factor activity establishment of vesicle localization negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway activation of MAPK activity stimulatory C-type lectin receptor signaling pathway apoptotic process inflammatory response immune response I-kappaB kinase/NF-kappaB signaling JNK cascade response to virus regulation of tumor necrosis factor-mediated signaling pathway TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway innate immune response T cell receptor signaling pathway stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway negative regulation of neuron death NF-kappaB import into nucleus

Process(es)

signal transducer activity protein binding protein domain specific binding ubiquitin protein ligase binding protein homodimerization activity peroxisome proliferator activated receptor binding metal ion binding protein heterodimerization activity K63-linked polyubiquitin binding linear polyubiquitin binding

IKK gamma Recombinant Rabbit Monoclonal Antibody (JA11-83)

Product Details

MA5-32682

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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