|Tested species reactivity||Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL1Ra, expressed in E.coli.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:500 - 1000|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||1:200|
|Immunohistochemistry (Frozen) (IHC (F))||1:400|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Radioimmune Assays (RIA)||1:8,000|
|Western Blot (WB)||1:400-1:800|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26495 detects IL1RA from primate samples. PA1-26495 will not react with rabbit or mouse.
PA1-26495 has been successfully used in ELISA, flow cytometry, immunohistochemistry (paraffin), immunohistochemistry (frozen), inhibition assay, radioimmune assay, and Western blot procedures.
The PA1-26495 immunogen is recombinant human IL1Ra, expressed in E.coli.
Endotoxin Content: <10 pg/ml by LAL method.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Cell communication Ligand-mediated signaling Immunity and defense B-cell- and antibody-mediated immunity