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|Tested species reactivity||Primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL1Ra, expressed in E.coli.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:500 - 1000|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||1:200|
|Immunohistochemistry (Frozen) (IHC (F))||1:400|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Radioimmune Assays (RIA)||1:8,000|
|Western Blot (WB)||1:400-1:800|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26495 detects IL1RA from primate samples. PA1-26495 will not react with rabbit or mouse.
PA1-26495 has been successfully used in ELISA, flow cytometry, immunohistochemistry (paraffin), immunohistochemistry (frozen), inhibition assay, radioimmune assay, and Western blot procedures.
The PA1-26495 immunogen is recombinant human IL1Ra, expressed in E.coli.
Endotoxin Content: <10 pg/ml by LAL method.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL1 inhibitor, IL1RN (IL1F3), interleukin-1 receptor antagonist protein, intracellular IL-1 receptor antagonist type II, intracellular interleukin-1 receptor antagonist (icIL-1ra), type II interleukin-1 receptor antagonist, Interleukin-1 Receptor Antagonist
IL-1ra, IL1F3, DIRA, IL-1ra3, IL1RA, IL-1RN, IRAP, ICIL-1RA, MVCD4
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Cell communication Ligand-mediated signaling Immunity and defense B-cell- and antibody-mediated immunity