M421A targets IL-1 alpha in ELISA applications and shows reactivity with Human samples.
The M421A immunogen is recombinant human IL-1 alpha.
M421A detects IL-1 alpha which has a predicted molecular weight of approximately 18 kDa.
The M421A IL1a antibody (clone ILA9-H18) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M420AB (biotinylated conjugate of clone ILA8-H12). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody M421A (clone ILA9-H18) and biotinylated antibody M420AB (clone ILA8-H12) have successfully been used in combination with recombinant IL1 alpha protein SIL1A in ELISA applications.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Hematopoietin-1; IL-1 alpha; interleukin 1, alpha; Interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
Gene Aliases: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552