|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant mouse IL-1 beta|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM425B targets IL-1 beta in WB and ELISA applications and shows reactivity with mouse samples.
The MM425B immunogen is recombinant mouse IL-1 beta.
MM425B detects IL-1 beta which has a predicted molecular weight of approximately 17 kDa.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Upregulated IL-1ß in dysferlin-deficient muscle attenuates regeneration by blunting the response to pro-inflammatory macrophages.
MM425B was used in blocking or activating experiment to study attenuation of regeneration by blunting the response to pro-inflammatory macrophages due to upregulation of IL-1beta in dysferlin-deficient muscle
|Cohen TV,Many GM,Fleming BD,Gnocchi VF,Ghimbovschi S,Mosser DM,Hoffman EP,Partridge TA||Skeletal muscle (5:null)||2015|
Photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation.
MM425B was used in blocking or activating experiment to study the protective effect of photodynamic therapy against bacterial arthritis and the mechanism involved
|Tanaka M,Mroz P,Dai T,Huang L,Morimoto Y,Kinoshita M,Yoshihara Y,Nemoto K,Shinomiya N,Seki S,Hamblin MR||PloS one (7:null)||2012|
Mediators of peripheral blood neutrophilia induced by photodynamic therapy of solid tumors.
MM425B was used in blocking/activating experiment to investigate the effect of photodynamic therapy on peripheral blood neutrophilia
|Cecic I,Korbelik M||Cancer letters (183:43)||2002|
Neutralization of interleukin-1 beta activity in vivo with a monoclonal antibody alleviates collagen-induced arthritis in DBA/1 mice and prevents the associated acute-phase response.
MM425B was used in blocking/activating experiment to investigate the effect of interleukin 1 beta inhibition for the therapy of arthritis in DBA/1 mice
|Geiger T,Towbin H,Cosenti-Vargas A,Zingel O,Arnold J,Rordorf C,Glatt M,Vosbeck K||Clinical and experimental rheumatology (11:515)||1994|
Inhibition of endoplasmic reticulum stress alleviates lipopolysaccharide-induced lung inflammation through modulation of NF-¿B/HIF-1¿ signaling pathway.
MM425B was used in western blot to study the mechanism by which inhibition of endoplasmic reticulum stress reduces lung inflammation induced by LPS
|Kim HJ,Jeong JS,Kim SR,Park SY,Chae HJ,Lee YC||Scientific reports (3:null)||2013|
Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.
MM425B was used in immunohistochemistry to study the effect of interleukin 1 beta on protection against Staphylococcus aureus
|Miller LS,Pietras EM,Uricchio LH,Hirano K,Rao S,Lin H,O'Connell RM,Iwakura Y,Cheung AL,Cheng G,Modlin RL||Journal of immunology (Baltimore, Md. : 1950) (179:6933)||2007|
Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of gamma delta T cells.
MM425B was used in immunohistochemistry to develop a neurocysticercosis animal model
|Cardona AE,Restrepo BI,Jaramillo JM,Teale JM||Journal of immunology (Baltimore, Md. : 1950) (162:995)||1999|
Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness.
MM425B was used in ELISA to investigate the role of TLR2 in the interferon-gamma responses
|Johnson DR,O'Connor JC,Dantzer R,Freund GG||Proceedings of the National Academy of Sciences of the United States of America (102:15184)||2005|
Role of caspase 1 in murine antibacterial host defenses and lethal endotoxemia.
MM425B was used in ELISA to study the role of caspase 1 in septic responses
|Joshi VD,Kalvakolanu DV,Hebel JR,Hasday JD,Cross AS||Infection and immunity (70:6896)||2002|
The CY domain of the Fcgamma RIa alpha-chain (CD64) alters gamma-chain tyrosine-based signaling and phagocytosis.
MM425B was used in ELISA to assess the interleukin-1beta production in transfected P388D1 cells
|Edberg JC,Qin H,Gibson AW,Yee AM,Redecha PB,Indik ZK,Schreiber AD,Kimberly RP||The Journal of biological chemistry (277:41287)||2002|
IL-18 levels and the outcome of innate immune response to lipopolysaccharide: importance of a positive feedback loop with caspase-1 in IL-18 expression.
MM425B was used in ELISA to investigate the mechanism for the innate immune response to lipopolysaccharide
|Joshi VD,Kalvakolanu DV,Hasday JD,Hebel RJ,Cross AS||Journal of immunology (Baltimore, Md. : 1950) (169:2536)||2002|
IFN-gamma inhibits lipopolysaccharide-induced interleukin-1 beta in primary murine macrophages via a Stat1-dependent pathway.
MM425B was used in ELISA to study the role of interferon gamma in LPS-induced interleukin 1 beta in primary murine macrophages
|De Boer ML,Hu J,Kalvakolanu DV,Hasday JD,Cross AS||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (21:485)||2001|
Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction.
MM425B was used in ELISA to study the effect of exogenous interleukin 4 on the cartilage repairment
|Lubberts E,Joosten LA,van Den Bersselaar L,Helsen MM,Bakker AC,van Meurs JB,Graham FL,Richards CD,van Den Berg WB||Journal of immunology (Baltimore, Md. : 1950) (163:4546)||1999|
Kinetic study of host defense and inflammatory response to Aspergillus fumigatus in steroid-induced immunosuppressed mice.
MM425b was used in ELISA to investigate the changes of host defense and inflammatory response to Aspergillus fumigatus in steroid-induced immunosuppressed mice
|Duong M,Ouellet N,Simard M,Bergeron Y,Olivier M,Bergeron MG||The Journal of infectious diseases (178:1472)||1998|