|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant mouse IL-1 beta|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM425BB targets IL-1 beta in ELISA, and WB applications and shows reactivity with mouse samples.
The MM425BB immunogen is recombinant mouse IL-1 beta.
MM425BB detects IL-1 beta which has a predicted molecular weight of approximately 17 kDa.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.
MM425BB was used in immunohistochemistry to study the effect of interleukin 1 beta on protection against Staphylococcus aureus
|Miller LS,Pietras EM,Uricchio LH,Hirano K,Rao S,Lin H,O'Connell RM,Iwakura Y,Cheung AL,Cheng G,Modlin RL||Journal of immunology (Baltimore, Md. : 1950) (179:6933)||2007|
Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of gamma delta T cells.
MM425BB was used in immunohistochemistry to develop a neurocysticercosis animal model
|Cardona AE,Restrepo BI,Jaramillo JM,Teale JM||Journal of immunology (Baltimore, Md. : 1950) (162:995)||1999|
IL-1beta-mediated innate immunity is amplified in the db/db mouse model of type 2 diabetes.
MM425BB was used in ELISA to study the interleukin 1beta-mediated innate immunity in the db/db mouse model of type 2 diabetes
|O'Connor JC,Satpathy A,Hartman ME,Horvath EM,Kelley KW,Dantzer R,Johnson RW,Freund GG||Journal of immunology (Baltimore, Md. : 1950) (174:4991)||2005|
Stimulus-dependent requirement for granulocyte-macrophage colony-stimulating factor in inflammation.
MM425BB was used in ELISA to investigate the role of granulocyte-macrophage colony-stimulating factor in inflammation
|Cook AD,Braine EL,Hamilton JA||Journal of immunology (Baltimore, Md. : 1950) (173:4643)||2004|
Sleep-deprived mice show altered cytokine production manifest by perturbations in serum IL-1ra, TNFa, and IL-6 levels.
MM425BB was used in ELISA to investigate the changes of cytokine expression in mice with sleep disruption
|Hu J,Chen Z,Gorczynski CP,Gorczynski LY,Kai Y,Lee L,Manuel J,Gorczynski RM||Brain, behavior, and immunity (17:498)||2003|
Essential role for Ca2+ in regulation of IL-1beta secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells.
MM425BB was used in ELISA to investigate the role of calcium ion in P2X7 nucleotide receptor-mediated interleukin 1 beta secretion
|Gudipaty L,Munetz J,Verhoef PA,Dubyak GR||American journal of physiology. Cell physiology (285:C286)||2003|
The CY domain of the Fcgamma RIa alpha-chain (CD64) alters gamma-chain tyrosine-based signaling and phagocytosis.
MM425BB was used in ELISA to study the mechanism for the gamma-chain tyrosine-based signaling and phagocytosis
|Edberg JC,Qin H,Gibson AW,Yee AM,Redecha PB,Indik ZK,Schreiber AD,Kimberly RP||The Journal of biological chemistry (277:41287)||2002|
IFN-gamma inhibits lipopolysaccharide-induced interleukin-1 beta in primary murine macrophages via a Stat1-dependent pathway.
MM425BB was used in ELISA to study the role of interferon-gamma in LPS-induced interleukin-1beta in primary murine macrophages
|De Boer ML,Hu J,Kalvakolanu DV,Hasday JD,Cross AS||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (21:485)||2001|
Mediators of peripheral blood neutrophilia induced by photodynamic therapy of solid tumors.
MM425BB was used in blocking/activating experiment to investigate the effect of photodynamic therapy on peripheral blood neutrophilia
|Cecic I,Korbelik M||Cancer letters (183:43)||2002|