Invitrogen

IL-1 beta Monoclonal Antibody (1400.24.17)

2 Published Figures

View all (1,842) IL-1 beta antibodies

Create Citation

Create Citation

Product Image Gallery

IL-1 beta Antibody — Published figure using IL-1 beta monoclonal antibody (Product # MM425B)

IL-1 beta Antibody — Figure 5 Effects of 4-PBA on the levels of IFN-gamma, TNF-alpha, IL-1beta, ICAM-1, KC, and VEGF protein in lung of LPS-treated mice. Sampling was performed at 48 hours after LPS treatment in 0.9% NaCl solution (saline)-treated mice administered with drug vehicle (PBS) (SAL+PBS), LPS-treated mice administered with PBS (LPS+PBS), or LPS-treated mice administered with 4-PBA (LPS+PBA). Representative Western blots of IFN-gamma (a), TNF-alpha (c), IL-1beta (e), and ICAM-1 (g) in the lung tissues and of KC (i) and VEGF (k) in the BAL fluids. Analyses of band intensity on films are presented as the relative ratio of IFN-gamma (b), TNF-alpha (d), IL-1beta (f), and ICAM-1 (h) to actin, while in case of KC and VEGF, analyses of band intensity on films are presented as the relative ratio of the level of KC (j) and VEGF (l) in each group to the level in SAL+PBS. The relative ratio of each protein in the lung tissues of SAL+PBS is arbitrarily presented as 1. Bars represent mean +- SEM from 8 mice per group. # P < 0.05 versus SAL+PBS; * P < 0.05 versus LPS+PBS.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay Dependent

Western Blot (WB)

Assay Dependent

Published Applications

Immunofluorescence (IF)

See 1 publication below

Neutralization (Neu)

See 5 publications below

Western Blot (WB)

See 1 publication below

Immunohistochemistry (IHC)

See 2 publications below

ELISA (ELISA)

See 7 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1400.24.17

Immunogen

Recombinant mouse IL-1 beta

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

RRID

AB_223529

Product Specific Information

MM425B targets IL-1 beta in WB and ELISA applications and shows reactivity with mouse samples.

The MM425B immunogen is recombinant mouse IL-1 beta.

MM425B detects IL-1 beta which has a predicted molecular weight of approximately 17 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: IL-1 beta; IL-1beta; Interleukin-1 beta

Gene Aliases: Il-1b; IL-1beta; Il1b

UniProt ID: (Mouse) P10749

Entrez Gene ID: (Mouse) 16176

Molecular Function: cytokine interleukin superfamily signaling molecule

negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation positive regulation of protein phosphorylation inflammatory response immune response positive regulation of cytosolic calcium ion concentration aging memory negative regulation of cell proliferation response to carbohydrate positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of glucose transport positive regulation of cell death negative regulation of glutamate secretion cytokine-mediated signaling pathway hyaluronan biosynthetic process neutrophil chemotaxis sequestering of triglyceride positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to lipopolysaccharide interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade positive regulation of immature T cell proliferation in thymus response to ATP positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity cellular response to drug positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of astrocyte differentiation positive regulation of phagocytosis negative regulation of neurogenesis regulation of insulin secretion leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of cell division positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to organic substance cellular response to organic cyclic compound positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Process(es)

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding