Invitrogen

IL-1 beta Monoclonal Antibody (1400.24.17)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

20 References

View all (80) IL-1 beta antibodies

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IL-1 beta Antibody in Western Blot (WB) — Western blot was performed using Anti-IL-1 beta Monoclonal Antibody (1400.24.17) (Product # MM425B) and a 35 kDa band corresponding to Interleukin-1 beta was observed in RAW 264.7, upon treatment with Lipopolysaccharide. Whole cell extracts (30 µg lysate) of RAW 264.7 (Lane 1), RAW 264.7 (Lipopolysaccharide (100 ng/mL), 24 hours) (Lane 2), THP-1 (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

IL-1 beta Antibody in Immunohistochemistry, Neutralization (IHC, Neu) — Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Immunohistochemistry and Neutralization

IL-1 beta Antibody in Neutralization (Neu) — Fig. 9 Upregulated IL-1beta inhibits muscle differentiation in A/J myoblasts. a Quantitation of myotube fusion in WT or A/J myoblasts treated with indicated concentrations of IL-1beta for 72 h. Percent myotube fusion was calculated from MyHC-positive immunostaining. b Quantitation of MyoD-positive cells are shown as percent of total nuclei in WT or A/J myoblast cultures treated with indicated concentrations of IL-1beta for 72 h. c Immunofluorescence staining of myoblast cultures after treatment with an IL-1beta blocking antibody (IL-1beta mAb). WT and A/J cultures were plated at equal density and treated with 4 ng/ml of IL-1beta mAb for 72 h. Control cultures were treated with equimolar concentrations of mouse IgG. Fixed cultures were stained with anti-MyoD (green) and anti-MyHC (red) antibodies. d Quantitation of myotube fusion in treated cultures. e Quantitation of MyoD-positive cells in treated cultures. f - h Gene expression of MyoD ( f ), Myf5 ( g ), and myogenin ( h ) determined by qRT-PCR in cultures treated with indicated concentrations of IL-1beta mAb. n = 3. Data are shown as means +- SEM. * P < 0.05; ** P < 0.01; *** P < 0.005

IL-1 beta Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using IL-1 beta Monoclonal Antibody (1400.24.17) (Product # MM425B), shows an induction of expression in RAW 264.7 upon treatment with Lipopolysaccharide. {TM}

Product Details

MM425B

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
ELISA (ELISA)	Assay-dependent	View 8 publications 8 publications
Neutralization (Neu)	-	View 5 publications 5 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	1400.24.17
Immunogen	Recombinant mouse IL-1 beta
Conjugate	Unconjugated Biotin
Form	Liquid
Concentration	1.0 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20°C
RRID	AB_223529

Product Specific Information

MM425B targets IL-1 beta in WB and ELISA applications and shows reactivity with mouse samples.

The MM425B immunogen is recombinant mouse IL-1 beta.

MM425B detects IL-1 beta which has a predicted molecular weight of approximately 17 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Huaier extract suppresses non-small cell lung cancer progression through activating NLRP3-dependent pyroptosis.

Anatomical record (Hoboken, N.J. : 2007)

Xie J,Zhuan B,Wang H,Wang Y,Wang X,Yuan Q,Yang Z

Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Dexamethasone Improves Wound Healing by Decreased Inflammation and Increased Vasculogenesis in Mouse Skin Frostbite Model.

Wilderness & environmental medicine

Tu H,Zhang D,Barksdale AN,Wadman MC,Muelleman RL,Li YL

MM425B was used in Western Blotting to identify that dexamethasone improved wound healing in frostbitten skin and demonstrated both anti-inflammatory effects and stimulation of vasculogenesis in mice.

Tue Dec 01 00:00:00 EST 2020

Extravascular gelation shrinkage-derived internal stress enables tumor starvation therapy with suppressed metastasis and recurrence.

Nature communications

Zhang K,Fang Y,He Y,Yin H,Guan X,Pu Y,Zhou B,Yue W,Ren W,Du D,Li H,Liu C,Sun L,Chen Y,Xu H

MM425B was used in an ELISA assay to establish an extravascular gelation shrinkage-derived internal stress strategy for squeezing and narrowing blood vessels, occluding blood & nutrition supply, reducing vascular density, inducing hypoxia and apoptosis and eventually realizing starvation therapy of malignancies.

Tue Nov 26 00:00:00 EST 2019

Chemerin-induced macrophages pyroptosis in fetal brain tissue leads to cognitive disorder in offspring of diabetic dams.

Journal of neuroinflammation

Liang Z,Han L,Sun D,Chen Y,Wu Q,Zhang L,Zhou M,Chen D

Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Western Blot

Sat Nov 16 00:00:00 EST 2019

Obesity-Induced Inflammation and Desmoplasia Promote Pancreatic Cancer Progression and Resistance to Chemotherapy.

Cancer discovery

Incio J,Liu H,Suboj P,Chin SM,Chen IX,Pinter M,Ng MR,Nia HT,Grahovac J,Kao S,Babykutty S,Huang Y,Jung K,Rahbari NN,Han X,Chauhan VP,Martin JD,Kahn J,Huang P,Desphande V,Michaelson J,Michelakos TP,Ferrone CR,Soares R,Boucher Y,Fukumura D,Jain RK

Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Obesity-Induced Inflammation and Desmoplasia Promote Pancreatic Cancer Progression and Resistance to Chemotherapy.

Cancer discovery

Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Upregulated IL-1β in dysferlin-deficient muscle attenuates regeneration by blunting the response to pro-inflammatory macrophages.

Skeletal muscle

Cohen TV,Many GM,Fleming BD,Gnocchi VF,Ghimbovschi S,Mosser DM,Hoffman EP,Partridge TA

Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Neutralization

Fri Aug 07 00:00:00 EDT 2015

Inhibition of endoplasmic reticulum stress alleviates lipopolysaccharide-induced lung inflammation through modulation of NF-κB/HIF-1α signaling pathway.

Scientific reports

Kim HJ,Jeong JS,Kim SR,Park SY,Chae HJ,Lee YC

Published figure using IL-1 beta monoclonal antibody (Product # MM425B) in Western Blot

Wed Jul 03 00:00:00 EDT 2013

Photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation.

PloS one

Tanaka M,Mroz P,Dai T,Huang L,Morimoto Y,Kinoshita M,Yoshihara Y,Nemoto K,Shinomiya N,Seki S,Hamblin MR

MM425B was used in blocking or activating experiment to study the protective effect of photodynamic therapy against bacterial arthritis and the mechanism involved

Fri Jan 04 00:00:00 EST 2013

Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Miller LS,Pietras EM,Uricchio LH,Hirano K,Rao S,Lin H,O'Connell RM,Iwakura Y,Cheung AL,Cheng G,Modlin RL

MM425B was used in immunohistochemistry to study the effect of interleukin 1 beta on protection against Staphylococcus aureus

Thu Nov 15 00:00:00 EST 2007

Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness.

Proceedings of the National Academy of Sciences of the United States of America

Johnson DR,O'Connor JC,Dantzer R,Freund GG

MM425B was used in ELISA to investigate the role of TLR2 in the interferon-gamma responses

Tue Oct 18 00:00:00 EDT 2005

Role of caspase 1 in murine antibacterial host defenses and lethal endotoxemia.

Infection and immunity

Joshi VD,Kalvakolanu DV,Hebel JR,Hasday JD,Cross AS

MM425B was used in ELISA to study the role of caspase 1 in septic responses

Sun Dec 01 00:00:00 EST 2002

The CY domain of the Fcgamma RIa alpha-chain (CD64) alters gamma-chain tyrosine-based signaling and phagocytosis.

The Journal of biological chemistry

Edberg JC,Qin H,Gibson AW,Yee AM,Redecha PB,Indik ZK,Schreiber AD,Kimberly RP

MM425B was used in ELISA to assess the interleukin-1beta production in transfected P388D1 cells

Fri Oct 25 00:00:00 EDT 2002

Mediators of peripheral blood neutrophilia induced by photodynamic therapy of solid tumors.

Cancer letters

Cecic I,Korbelik M

MM425B was used in blocking/activating experiment to investigate the effect of photodynamic therapy on peripheral blood neutrophilia

Sun Sep 08 00:00:00 EDT 2002

IL-18 levels and the outcome of innate immune response to lipopolysaccharide: importance of a positive feedback loop with caspase-1 in IL-18 expression.

Journal of immunology (Baltimore, Md. : 1950)

Joshi VD,Kalvakolanu DV,Hasday JD,Hebel RJ,Cross AS

MM425B was used in ELISA to investigate the mechanism for the innate immune response to lipopolysaccharide

Sun Sep 01 00:00:00 EDT 2002

IFN-gamma inhibits lipopolysaccharide-induced interleukin-1 beta in primary murine macrophages via a Stat1-dependent pathway.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

De Boer ML,Hu J,Kalvakolanu DV,Hasday JD,Cross AS

MM425B was used in ELISA to study the role of interferon gamma in LPS-induced interleukin 1 beta in primary murine macrophages

Sun Jul 01 00:00:00 EDT 2001

Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction.

Journal of immunology (Baltimore, Md. : 1950)

Lubberts E,Joosten LA,van Den Bersselaar L,Helsen MM,Bakker AC,van Meurs JB,Graham FL,Richards CD,van Den Berg WB

MM425B was used in ELISA to study the effect of exogenous interleukin 4 on the cartilage repairment

Fri Oct 15 00:00:00 EDT 1999

Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of gamma delta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Cardona AE,Restrepo BI,Jaramillo JM,Teale JM

MM425B was used in immunohistochemistry to develop a neurocysticercosis animal model

Fri Jan 15 00:00:00 EST 1999

Kinetic study of host defense and inflammatory response to Aspergillus fumigatus in steroid-induced immunosuppressed mice.

The Journal of infectious diseases

Duong M,Ouellet N,Simard M,Bergeron Y,Olivier M,Bergeron MG

MM425b was used in ELISA to investigate the changes of host defense and inflammatory response to Aspergillus fumigatus in steroid-induced immunosuppressed mice

Sun Nov 01 00:00:00 EST 1998

Neutralization of interleukin-1 beta activity in vivo with a monoclonal antibody alleviates collagen-induced arthritis in DBA/1 mice and prevents the associated acute-phase response.

Clinical and experimental rheumatology

Geiger T,Towbin H,Cosenti-Vargas A,Zingel O,Arnold J,Rordorf C,Glatt M,Vosbeck K

MM425B was used in blocking/activating experiment to investigate the effect of interleukin 1 beta inhibition for the therapy of arthritis in DBA/1 mice

Mon Feb 07 00:00:00 EST 1994

Bioinformatics

Protein Aliases: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta

Gene Aliases: IL-1; Il-1b; IL-1beta; IL1-BETA; IL1B; IL1F2

UniProt ID: (Human) P01584, (Mouse) P10749

Entrez Gene ID: (Human) 3553, (Mouse) 16176

Molecular Function: interleukin superfamily