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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
Assay-dependent | - |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
ELISA (ELISA) |
Assay-dependent | View 8 publications 8 publications |
Neutralization (Neu) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
1400.24.17 |
Immunogen |
Recombinant mouse IL-1 beta |
Conjugate |
Biotin
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS with 4% BSA |
Contains |
proprietary preservative |
Storage conditions |
-20°C |
RRID |
AB_223603 |
MM425BB targets IL-1 beta in ELISA, and WB applications and shows reactivity with mouse samples.
The MM425BB immunogen is recombinant mouse IL-1 beta.
MM425BB detects IL-1 beta which has a predicted molecular weight of approximately 17 kDa.
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta
Gene Aliases: Il-1b; IL-1beta; Il1b
UniProt ID: (Mouse) P10749
Entrez Gene ID: (Mouse) 16176
Molecular Function:
interleukin superfamily
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