Invitrogen

IL-1 beta Monoclonal Antibody (30311)

View all (71) IL-1 beta antibodies

Questions & Answers

Questions & Answers

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IL-1 beta Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouse IL-1ß/IL-1F2 stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by recombinant mouse IL-1ß/IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-mouse IL-1ß/IL-1F2 Monoclonal Antibody (Product # MA5-23721). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Product Details

MA5-23721

Applications	Tested Dilution	Publications
ELISA (ELISA)	0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)	-
Neutralization (Neu)	1-5 µg/mL	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Rat / IgG1
Class	Monoclonal
Type	Antibody
Clone	30311
Immunogen	E. coli-derived recombinant mouse IL-1 beta/IL-1F2 Val118-Ser269 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.5 mg/mL
Purification	Protein A/G
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2609520

Product Specific Information

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

Gene Aliases: Il-1b; IL-1beta; Il1b

UniProt ID: (Mouse) P10749

Entrez Gene ID: (Mouse) 16176

Molecular Function: interleukin superfamily

negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation positive regulation of protein phosphorylation inflammatory response immune response positive regulation of cytosolic calcium ion concentration aging memory negative regulation of cell proliferation response to carbohydrate positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of glucose transport positive regulation of cell death negative regulation of glutamate secretion cytokine-mediated signaling pathway hyaluronan biosynthetic process neutrophil chemotaxis sequestering of triglyceride positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to lipopolysaccharide interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade positive regulation of immature T cell proliferation in thymus response to ATP positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity cellular response to drug positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of astrocyte differentiation positive regulation of phagocytosis negative regulation of neurogenesis regulation of insulin secretion leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of cell division positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to organic substance cellular response to organic cyclic compound positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Process(es)

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding