Normal human peripheral blood cells were stimulated with LPS in the presence of Brefeldin A for 4 hours. The cells were surface stained with Anti-Human CD14 PE and intracellularly stained with Anti-Human IL-1 beta FITC (top). The cells were surface stained with Anti-Human CD14 FITC and intracellularly stained with Anti-Human IL-1 beta PE (A18465, bottom).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human interleukin-1 beta|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
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