Sandwich ELISA analysis of Mouse anti-Human IL-1beta biotinylated monoclonal antibody (Product #M420BB) was performed using the Thermo Scientific ELISA kit EH2IL1B by loading 50µl per well of Human IL-1beta recombinant protein (Product #SIL1B) in quadruplicate at 400, 160, 64, 25.6, 10.24 and 0 pg/ml across a 5µg/ml Mouse anti-Human IL-1beta (Product #M421B) pre-coated plate and incubated for 3 hours at room temperature. The plate was washed then incubated with 50µl per well of Mouse anti-Human IL-1beta biotinylated antibody (Product #M420BB) in quadruplicate at 0.07µg/ml for 3 hours at room temperature. The plate was washed and incubated with 100µl per well of Ultra Streptavidin-HRP (Product #N504) in all test wells at 1:3000 for 30 minutes at room temperature. The plate was washed then incubated with 100µl per well of Ultra TMB substrate (Product #34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human IL-1 beta|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 8 publications below|
M420BB targets IL-1 beta in ELISA applications and shows reactivity with Human samples.
The M420BB immunogen is recombinant human IL-1 beta.
M420BB detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.
The M420BB IL1b antibody (biotinylated conjugate of clone ILB1-H6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M421B (clone ILB1-H67). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M420BB (clone ILB1-H6) and antibody M421B (clone ILB1-H67) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
M420BB was used in ELISA to study the role of Th1 responses to beta amyloid in Down syndrome and Alzheimer disease
|Loewenbrueck KF,Tigno-Aranjuez JT,Boehm BO,Lehmann PV,Tary-Lehmann M||Neurobiology of aging (31:1732)||2010|
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
M420BB was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera
|Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F||Journal of immunological methods (316:1)||2006|
Relationship between antipyretic effects and cytokine levels in uncomplicated falciparum malaria during different treatment regimes.
M420BB was used in ELISA to investigate the effects of antipyretics on cytokine levels in uncomplicated falciparum malaria
|Hugosson E,Montgomery SM,Premji Z,Troye-Blomberg M,Björkman A||Acta tropica (99:75)||2006|
Determination of the inflammatory potential of bioaerosols from a duck-fattening unit by using a limulus amebocyte lysate assay and human whole blood cytokine response.
M420BB was used in ELISA to investigate the reliability of the whole blood assay for the detection of immune response induced by bioaerosols
|Zucker BA,Scharf P,Kersten C||Journal of veterinary medicine. B, Infectious diseases and veterinary public health (53:176)||2006|
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
M420BB was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients
|Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M||Rheumatology (Oxford, England) (43:960)||2004|
Essential role for Ca2+ in regulation of IL-1beta secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells.
M420BB was used in ELISA to investigate the role of calcium ion in P2X7 nucleotide receptor-mediated interleukin 1 beta secretion
|Gudipaty L,Munetz J,Verhoef PA,Dubyak GR||American journal of physiology. Cell physiology (285:C286)||2003|
HIV-1 coat protein gp120 stimulates interleukin-1beta secretion from human neuroblastoma cells: evidence for a role in the mechanism of cell death.
M420BB was used in ELISA to investigate the role of interleukin 1 beta during the cell death induced by HIV infection
|Corasaniti MT,Bilotta A,Strongoli MC,Navarra M,Bagetta G,Di Renzo G||British journal of pharmacology (134:1344)||2001|
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
M420BB was used in ELISA to investigate the role of mast cells/basophils in dengue pathogenesis
|King CA,Marshall JS,Alshurafa H,Anderson R||Journal of virology (74:7146)||2000|